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  4. Recombinant Bacillus subtilis Putative 8-oxo-dGTP diphosphatase YtkD (ytkD)

Recombinant Bacillus subtilis Putative 8-oxo-dGTP diphosphatase YtkD (ytkD)

  • 中文名称：
    枯草芽孢杆菌ytkD重组蛋白
  • 货号：
    CSB-YP522043BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌ytkD重组蛋白
  • 货号：
    CSB-EP522043BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌ytkD重组蛋白
  • 货号：
    CSB-EP522043BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌ytkD重组蛋白
  • 货号：
    CSB-BP522043BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌ytkD重组蛋白
  • 货号：
    CSB-MP522043BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ytkD
  • Uniprot No.：
  • 别名：
    ytkD; mutTA; BSU30630; Putative 8-oxo-dGTP diphosphatase YtkD; 8-oxo-dGTPase; EC 3.6.1.55; 7,8-dihydro-8-oxoguanine-triphosphatase; dGTP pyrophosphohydrolase
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-158
  • 氨基酸序列
    MYEFKDYYQN TVQLSFDDQP FSDSPKHVWV ICRFGGKWLL TEHEDRGYEF PGGKVEPMEC AEEAALREVK EETGARVKSL KYLGQYKVLG KEKVIVKNIY FADIEKLEKQ ADYFETKGPV LFHELPENLS RNKKFSFIMK DSVLPISLKK LKESGWIE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine, 8-oxo-dGTP) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. Functions, in conjunction with MutT, to protect vegetatively growing cells from DNA-damaging agents such as H(2)O(2) or t-BHP (t-butylhydroperoxide). The 2 proteins do not however protect spores. According to PubMed:15576788, phosphohydrolase that catalyzes the hydrolysis of all common nucleoside triphosphates as well as of the mutagenic analog 8-oxo-dGTP. The high catalytic efficiency on dGTP is in contrast to results from PubMed:14761999. According to PubMed:14761999, catalyzes the hydrolysis of 8-oxo-dGTP with a specific activity 413 times higher than that exhibited against dGTP. Preferentially catalyzes the hydrolysis of 8-oxo-dGTP and 8-oxo-GTP. According to PubMed:15576788, hydrolyzes nucleoside triphosphates in a stepwise fashion through the diphosphate to the monophosphate, releasing two molecules of inorganic orthophosphate.
  • 基因功能参考文献：
    1. characterized as a nucleoside triphosphatase member of nudix hydrolase superfamily and not an orthologue of E coli mutT. PMID: 15576788
    2. YtkD and MutT play an antimutator role and protect growing cells of B. subtilis against oxidizing agents PMID: 16513759
  • 蛋白家族：
    Nudix hydrolase family
  • 数据库链接：

    KEGG: bsu:BSU30630

    STRING: 224308.Bsubs1_010100016666