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  4. Recombinant Bacillus subtilis L-threonine dehydratase biosynthetic IlvA (ilvA)

Recombinant Bacillus subtilis L-threonine dehydratase biosynthetic IlvA (ilvA)

  • 中文名称：
    枯草芽孢杆菌ilvA重组蛋白
  • 货号：
    CSB-YP334342BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌ilvA重组蛋白
  • 货号：
    CSB-EP334342BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌ilvA重组蛋白
  • 货号：
    CSB-EP334342BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌ilvA重组蛋白
  • 货号：
    CSB-BP334342BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌ilvA重组蛋白
  • 货号：
    CSB-MP334342BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ilvA
  • Uniprot No.：
  • 别名：
    ilvA; BSU21770; L-threonine dehydratase biosynthetic IlvA; EC 4.3.1.19; Threonine deaminase
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-422
  • 氨基酸序列
    MKPLLKENSL IQVKHILKAH QNVKDVVIHT PLQRNDRLSE RYECNIYLKR EDLQVVRSFK LRGAYHKMKQ LSSEQTENGV VCASAGNHAQ GVAFSCKHLG IHGKIFMPST TPRQKVSQVE LFGKGFIDII LTGDTFDDVY KSAAECCEAE SRTFIHPFDD PDVMAGQGTL AVEILNDIDT EPHFLFASVG GGGLLSGVGT YLKNVSPDTK VIAVEPAGAA SYFESNKAGH VVTLDKIDKF VDGAAVKKIG EETFRTLETV VDDILLVPEG KVCTSILELY NECAVVAEPA GALSVAALDL YKDQIKGKNV VCVVSGGNND IGRMQEMKER SLIFEGLQHY FIVNFPQRAG ALREFLDEVL GPNDDITRFE YTKKNNKSNG PALVGIELQN KADYGPLIER MNKKPFHYVE VNKDEDLFHL LI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.
  • 蛋白家族：
    Serine/threonine dehydratase family
  • 数据库链接：

    KEGG: bsu:BSU21770

    STRING: 224308.Bsubs1_010100011991