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  4. Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH (ywfH)

Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH (ywfH)

  • 中文名称：
    Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH(ywfH),Yeast
  • 货号：
    CSB-YP328007BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH(ywfH),Yeast
  • 货号：
    CSB-EP328007BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH(ywfH),Yeast
  • 货号：
    CSB-EP328007BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH(ywfH),Yeast
  • 货号：
    CSB-BP328007BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Bacillus subtilis Bacilysin biosynthesis oxidoreductase ywfH(ywfH),Yeast
  • 货号：
    CSB-MP328007BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    bacG
  • Uniprot No.：
  • 别名：
    bacG; ywfH; BSU37680; ipa-86rNADPH-dependent reductase BacG; EC 1.3.1.-; Bacilysin biosynthesis oxidoreductase YwfH; H2HPP reductase
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-259
  • 氨基酸序列
    MSKRTAFVMG ASQGIGKAIA LKLADQHFSL VINSRNLDNI ESVKEDILAK HPEASVIVLA GDMSDQHTRA GIFQKIESQC GRLDVLINNI PGGAPDTFDN CNIEDMTATF TQKTVAYIDA IKRASSLMKQ NEFGRIINIV GNLWKEPGAN MFTNSMMNAA LINASKNISI QLAPHNITVN CLNPGFIATD RYHQFVENVM KKNSISKQKA EEQIASGIPM KRVGSAEETA ALAAFLASEE ASYITGQQIS ADGGSMKSI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Along with the bacABCDEF operon, BacG is involved in the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase. BacG catalyzes the stereoselective reduction of exocyclic-delta(3),delta(5)-dihydro-hydroxyphenylpyruvate (ex-H2HPP), adding a pro-S hydride equivalent to C4 position to yield tetrahydro-hydroxyphenylpyruvate (H4HPP). Although the 3Z,7R-ex-H2HPP isomer is kinetically disfavored by BacB and produced in a smaller quantity than 3E,7R-ex-H2HPP, it is the preferred substrate for the conjugate reduction reaction of BacG.
  • 基因功能参考文献：
    1. The crystal structure of YwfH was determined in three conformational states at different steps of catalysis, representing apo YwfH, the YwfH-NADPH complex and an apo-like form. PMID: 23519407
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Short-chain dehydrogenases/reductases (SDR) family
  • 数据库链接：

    KEGG: bsu:BSU37680

    STRING: 224308.Bsubs1_010100020356