Recombinant Bacillus licheniformis Subtilisin Carlsberg (apr)

Recombinant Bacillus licheniformis apr is an E.coli cell-expressed protein (Full Length of Mature Protein). E.coli expression systems are popular because bacteria are easy to culture, grow fast and produce high yields of recombinant protein. Traditional strategies for Bacillus licheniformis apr recombinant protein expression involve transforming the recombinant plasmid into the E. coli host. The cells were directly replicated, transcribed and translated, and IPTG was added to express the desired protein. Typically, the cells are then lysed to extract the expressed protein for subsequent purification. Also, many proteins become insoluble as inclusion bodies that are very difficult to recover, so mild conditions and subsequent cumbersome protein-refolding procedures are used to ensure the final provided protein is soluble.

apr (also known as subC) is a gene providing an instruction in making a protein named subtilisin carlsberg in Bacillus licheniformis. The protein encoded by this gene belongs to peptidase S8 family, a group of serine proteases that – like all serine proteases – initiate the nucleophilic attack on the peptide bond through a serine residue at the active site. The gene from Bacillus licheniformis has been isolated by molecular cloning using a mixture of synthetic oligonucleotides. The entire nucleotide sequence of the coding sequence as well as 5′ and 3′ flanking sequences have been determined.