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  4. Recombinant Autographa californica nuclear polyhedrosis virus FAD-linked sulfhydryl oxidase (P33)

Recombinant Autographa californica nuclear polyhedrosis virus FAD-linked sulfhydryl oxidase (P33)

  • 货号：
    CSB-YP334852ARA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP334852ARA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP334852ARA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP334852ARA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP334852ARA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    P33
  • Uniprot No.：
  • 别名：
    P33; ORF92FAD-linked sulfhydryl oxidase; p33; EC 1.8.3.2
  • 种属：
    Autographa californica nuclear polyhedrosis virus (AcMNPV)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-259
  • 氨基酸序列
    MIPLTPLFSR YKDSYLLYSF RLIDLLRASK STHLTKLLSS QATYLYHFAC LMKYKDIQKY EVQQLIEWAI NASPDMDLQQ FRIEFMDKTT ELNLRSCQPK SFTYTFTTIW DTMHFLSLII DDMVYTRDKS SLDFVMQQLK TMKVLFYNVF FILQCAMCRD HYMNVKGFII YHIELIEIAL DKEKYGTDIT FVDSYQQETA GADVAVVSNN MLMKNLMAYV SMTFHNHIND YKWIQRNKKP PAHYERMTWG EYKKLLNLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functional FAD-linked sulfhydryl oxidase that is required for infectious budded virion (BV) production and for the formation of enveloped occluded virion (ODV).
  • 基因功能参考文献：
    1. results showed that the three conserved regions, i.e., the active site, the dimer interface, and the R127-E183 salt bridge, are key regions of P33 and play significant roles in virus morphogenesis and oral infectivity PMID: 28904203
    2. The Trichoplusia ni single nucleopolyhedrovirus tn79 gene is able to support the replication of Autographa californica multiple nucleopolyhedrovirus lacking the ac92 gene. PMID: 25010286
    3. Thus, although Ac92 binds to Spodoptera frugiperda P53 during Autographa californica M nucleopolyhedrovirus replication and oxidizes SfP53 in vitro, s could not detect any effects of this interaction on virus replication. PMID: 24210115
    4. This novel assembly comprises a dimer of pseudodimers with a striking 40-degree kink in the interface helix between subunits. PMID: 21752922
    5. AC92 plays a key role in virion morphogenesis and is essential for the successful infection. PMID: 20965540

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  • 亚细胞定位：
    Host cytoplasm. Host nucleus.
  • 蛋白家族：
    Baculoviridae p33 family
  • 数据库链接：

    KEGG: vg:1403925