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  4. Recombinant Arabidopsis thaliana WUSCHEL-related homeobox 5 (WOX5)

Recombinant Arabidopsis thaliana WUSCHEL-related homeobox 5 (WOX5)

  • 货号：
    CSB-YP814116DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP814116DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP814116DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP814116DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP814116DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WOX5
  • Uniprot No.：
  • 别名：
    WOX5; At3g11260; F11B9.18; WUSCHEL-related homeobox 5
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-182
  • 氨基酸序列
    MSFSVKGRSL RGNNNGGTGT KCGRWNPTVE QLKILTDLFR AGLRTPTTDQ IQKISTELSF YGKIESKNVF YWFQNHKARE RQKRRKISID FDHHHHQPST RDVFEISEED CQEEEKVIET LQLFPVNSFE DSNSKVDKMR ARGNNQYREY IRETTTTSFS PYSSCGAEME HPPPLDLRLS FL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor, which may be involved in the specification and maintenance of the stem cells (QC cells) in the root apical meristem (RAM).
  • 基因功能参考文献：
    1. the expression switch from WOX11/12 to WOX5/7 is critical for initiation of the root primordium during de novo root organogenesis. PMID: 27784768
    2. WOX5 plays a role in root stem cell niche maintenance. PMID: 26440429
    3. WOX5 signals from the quiescent centre to promote columella stem cell (CSC) fate, while CLE40 is secreted from the differentiated columella cells to promote differentiation. PMID: 26019259
    4. ROW1 is essential for quiescent centre maintenance and for stem cell niche development through the repression of WOX5 in the proximal meristem. PMID: 25631790
    5. Results show that ACR4 phosphorylates WOX5 through four phosphorylation sites within the WOX5 protein sequence. PMID: 25756623
    6. WOX5 restrains cell division in the cells of the quiescent center, thereby establishing quiescence. In contrast, WOX5 and CYCD3;3/CYCD1;1 both promote cell proliferation in the nascent columella. PMID: 25127220
    7. WOX5-IAA17 feedback circuit is essential for the maintenance of auxin gradient in the root tip. PMID: 23939433
    8. The MKO1 gene plays an important role in the maintenance of the root apical meristem proliferative capacity and indeterminate root growth, which apparently acts independently of the SCR/SHR and WOX5 regulatory pathways. PMID: 21744091

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WUS homeobox family
  • 组织特异性：
    Specifically expressed in the central cells of a quiescent center (QC) of the root.
  • 数据库链接：

    KEGG: ath:AT3G11260

    STRING: 3702.AT3G11260.1

    UniGene: At.39787