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  4. Recombinant Arabidopsis thaliana WD-40 repeat-containing protein MSI1 (MSI1)

Recombinant Arabidopsis thaliana WD-40 repeat-containing protein MSI1 (MSI1)

  • 中文名称：
    拟南芥MSI1重组蛋白
  • 货号：
    CSB-YP522782DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥MSI1重组蛋白
  • 货号：
    CSB-EP522782DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥MSI1重组蛋白
  • 货号：
    CSB-EP522782DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥MSI1重组蛋白
  • 货号：
    CSB-BP522782DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥MSI1重组蛋白
  • 货号：
    CSB-MP522782DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MSI1
  • Uniprot No.：
  • 别名：
    MSI1; At5g58230; MCK7.10; Histone-binding protein MSI1; CAF-1 p48 homolog; Chromatin assembly factor 1 subunit MSI1; CAF-1 subunit MSI1; Protein MULTICOPY SUPPRESSOR OF IRA 1; AtMSI1; Protein medicis; WD-40 repeat-containing protein MSI1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-424
  • 氨基酸序列
    MGKDEEEMRG EIEERLINEE YKIWKKNTPF LYDLVITHAL EWPSLTVEWL PDREEPSGKD YSVQKMILGT HTSESEPNYL MLAQVQLPLD DTESEARQYD DDRSEFGGFG CATGKVQIIQ QINHDGEVNR ARYMPQNPFI IATKTVNAEV YVFDYSKHPS KPPLDGACNP DLKLRGHSSE GYGLSWSKFK QGHLLSGSDD AQICLWDINA TPKNKSLDAQ QIFKAHEGVV EDVAWHLRHE YLFGSVGDDQ YLLIWDLRSP SASKPVQSVV AHSMEVNCLA FNPFNEWVVA TGSTDKTVKL FDLRKLSTAL HTFDSHKEEV FQVGWNPKNE TILASCCLGR RLMVWDLSRI DEEQTVEDAE DGPPELLFIH GGHTSKISDF SWNPCEDWVI SSVAEDNILQ IWQMAENIYH DEDDAPGEEP SKAS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair, and the fertilization independent seed (FIS) complex, a polycomb group protein complex which is required to maintain the transcriptionally repressive state of homeotic genes throughout development. Required for several aspects of plant development including normal leaf expansion, correct development of flowers, normal endosperm development, repression of parthenogenetic seed development and repression of floral homeotic genes in leaf tissue. Also required for the recruitment of chromosomal DNA into heterochromatic chromocenters. Also involved in regulation of imprinted genes. Acts together with RBR1 to repress the expression of MET1 during female gametogenesis. This in turn activates expression of the imprinted genes FIS2 and FWA.
  • 基因功能参考文献：
    1. MSI1 is a novel gene which regulates flowering. PMID: 16554362
    2. These results reveal that the functions of FAS1, FAS2 and MSI1 are not restricted to meristems, but are also needed to control genome replication at multiple steps of development. PMID: 17021044
    3. Data suggest that the Polycomb group protein MSI1 functions in the female gametophyte to determine seed development in plants. PMID: 17855429
    4. Rb-dependent activation is mediated by direct transcriptional repression of MET1 during female gametogenesis. PMID: 18700816
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WD repeat RBAP46/RBAP48/MSI1 family
  • 组织特异性：
    Ubiquitously expressed. Strongly expressed in floral buds and flowers, the female gametophyte and the sporophytic tissue of the ovules. Also strongly expressed in developing embryos following fertilization.
  • 数据库链接：

    KEGG: ath:AT5G58230

    STRING: 3702.AT5G58230.1

    UniGene: At.49185