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  4. Recombinant Arabidopsis thaliana Two-component response regulator-like APRR9 (APRR9)

Recombinant Arabidopsis thaliana Two-component response regulator-like APRR9 (APRR9)

  • 货号：
    CSB-YP811853DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP811853DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP811853DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP811853DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP811853DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    APRR9
  • Uniprot No.：
  • 别名：
    APRR9; At2g46790; F19D11.7Two-component response regulator-like APRR9; Pseudo-response regulator 9
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-468
  • 氨基酸序列
    MGEIVVLSSD DGMETIKNRV KSSEVVQWEK YLPKTVLRVL LVESDYSTRQ IITALLRKCC YKVVAVSDGL AAWEVLKEKS HNIDLILTEL DLPSISGFAL LALVMEHEAC KNIPVIMMSS QDSIKMVLKC MLRGAADYLI KPMRKNELKN LWQHVWRRLT LRDDPTAHAQ SLPASQHNLE DTDETCEDSR YHSDQGSGAQ AINYNGHNKL MENGKSVDER DEFKETFDVT MDLIGGIDKR PDSIYKDKSR DECVGPELGL SLKRSCSVSF ENQDESKHQK LSLSDASAFS RFEESKSAEK AVVALEESTS GEPKTPTESH EKLRKVTSDQ GSATTSSNQE NIGSSSVSFR NQVLQSTVTN QKQDSPIPVE SNREKAASKE VEAGSQSTNE GIAGQSSSTE KPKEEESAKQ RWSRSQREAA LMKFRLKRKD RCFDKKVRYQ SRKKLAEQRP RVKGQFVRTV NSDASTKS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor of CCA1 and LHY, and positive regulator of LWD1 and LWD2 expression. Controls photoperiodic flowering response and temperature compensation. Involved in the positive and negative feedback loops of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. Regulated at the transcriptional level by a corepressor complex consisting of ELF4, ELF3, and LUX. APRR9, APRR7, and APRR5 coordinately act on the upstream region of the target genes to repress their expression from noon until midnight. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock.
  • 基因功能参考文献：
    1. PRR9, a core circadian component, is a key regulator of leaf senescence via positive regulation of ORE1 through a feed-forward pathway involving posttranscriptional regulation by miR164 and direct transcriptional regulation. PMID: 30065116
    2. Transcriptional co-regulators PRR9, PRR7 and PRR5 inhibit morning loops LHY and CCA1 expression by binding to their promoters. PMID: 24267177
    3. PPR9 regulates the promoter activity of LWD1/2. PMID: 21357491
    4. Results reveal a role of PRR7 and PRR9 in regulating CCA1 and LHY activities in response to ambient temperature. PMID: 21098730
    5. PRR9, PRR7, and PRR5 proteins are major transcriptional repressors of CCA1 and LHY and are thus essential for proper clock function. PMID: 20233950
    6. single prr7-3 or prr9-1 mutants exhibit modest period lengthening, but the prr7-3 prr9-1 double mutant shows dramatic and more than additive period lengthening in the light and becomes arrhythmic in constant darkness PMID: 15705949
    7. The APRR9 promoter contains at least two distinctive and separable regulatory cis-elements: an "L element" responsible for the light-induced expression, followed by an "R element" necessary for the fundamental rhythmic expression of the gene. PMID: 15725665
    8. PRR9/PRR7/PRR5 together act as period-controlling factors, and they play overlapping and distinctive roles close to (or within) the central oscillator in which the relative, PRR1/TOC1, plays an essential role PMID: 15767265
    9. The role of clock-associated genes PRR9, PRR7 and PRR5 are involved in activation of CONSTANS and CO-FLOWERING LOCUS T are reported. PMID: 17504813
    10. Data show that PRR9 polypeptides oscillate diurnally, and that they accumulate rapidly in response to light, and further suggest that the presence of PRR9 polypeptides is controlled through proteasome-mediated programmed degradation in the dark. PMID: 17890242

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ARR-like family
  • 数据库链接：

    KEGG: ath:AT2G46790

    STRING: 3702.AT2G46790.1

    UniGene: At.1675