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  4. Recombinant Arabidopsis thaliana Transcriptional activator DEMETER (DME), partial

Recombinant Arabidopsis thaliana Transcriptional activator DEMETER (DME), partial

  • 中文名称：
    拟南芥DME重组蛋白
  • 货号：
    CSB-EP839701DOA
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    DME
  • Uniprot No.：
  • 别名：
    DME; At5g04560/At5g04570/At5g04580; T32M21.160/T32M21.170/T32M21.180Transcriptional activator DEMETER; EC 3.2.2.-; DNA glycosylase-related protein DME
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    51.4kDa
  • 表达区域：
    1-320aa
  • 氨基酸序列
    MNSRADPGDRYFRVPLENQTQQEFMGSWIPFTPKKPRSSLMVDERVINQDLNGFPGGEFVDRGFCNTGVDHNGVFDHGAHQGVTNLSMMINSLAGSHAQAWSNSERDLLGRSEVTSPLAPVIRNTTGNVEPVNGNFTSDVGMVNGPFTQSGTSQAGYNEFELDDLLNPDQMPFSFTSLLSGGDSLFKVRQYGPPACNKPLYNLNSPIRREAVGSVCESSFQYVPSTPSLFRTGEKTGFLEQIVTTTGHEIPEPKSDKSMQSIMDSSAVNATEATEQNDGSRQDVLEFDLNKTPQQKPSKRKRKFMPKVVVEGKPKRKPRK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator involved in gene imprinting. Catalyzes the release of 5-methylcytosine (5-meC) from DNA by a glycosylase/lyase mechanism. Allows the expression of the maternal copy of the imprinted MEA gene before fertilization, possibly by antagonizing or suppressing DNA methylation on target promoter. Probably acts by nicking the MEA promoter. Required for stable reproducible patterns of floral and vegetative development.
  • 基因功能参考文献：
    1. DME and ROS1 have a significant 5-hydroxymethylcytosine (5hmC) excision activity in vitro, although the study detected no 5hmC in Arabidopsis, suggesting that it is very unlikely for plants to utilize 5hmC as a DNA demethylation intermediate. PMID: 24661881
    2. Histone H1 is involved in DME-mediated DNA methylation and gene regulation at imprinted loci. PMID: 22519754
    3. DME is active and demethylates similar genes and transposons in the genomes of the vegetative and central cells in the male and female gametophytes PMID: 21518889
    4. Analyses indicate that AtLIG1 acts downstream of DME. The removal of methylated cytosine residues by DME involves the creation of DNA single-strand breaks and our results suggest that AtLIG1 repairs these breaks. PMID: 20023162
    5. Demethylation by DEMETER is necessary for genomic imprinting and defines the genomic methylation profile of Arabidopsis genes. PMID: 17409185
    6. Gene ontology analysis identified three molecular functions enriched in the DME-inducible RNA list: DNA or RNA binding, kinase activity, and transcription factor activity. PMID: 17964287
    7. control DNA methylation in Arabidopsis thaliana.[review] PMID: 18536342

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA glycosylase family, DEMETER subfamily
  • 组织特异性：
    Mainly expressed in immature flower buds, then decreases as the flower matures. Expressed in the ovule carpels, but not expressed in pollen stamens. Expressed in developing and mature ovules (stages 12-14), then strongly decreases after fertilization.
  • 数据库链接：

    KEGG: ath:AT5G04560

    STRING: 3702.AT5G04560.2

    UniGene: At.33104