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  4. Recombinant Arabidopsis thaliana Transcription factor PIF4 (PIF4)

Recombinant Arabidopsis thaliana Transcription factor PIF4 (PIF4)

  • 中文名称：
    拟南芥PIF4重组蛋白
  • 货号：
    CSB-YP848762DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PIF4重组蛋白
  • 货号：
    CSB-EP848762DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PIF4重组蛋白
  • 货号：
    CSB-EP848762DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PIF4重组蛋白
  • 货号：
    CSB-BP848762DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PIF4重组蛋白
  • 货号：
    CSB-MP848762DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PIF4
  • Uniprot No.：
  • 别名：
    PIF4; BHLH9; EN102; SRL2; At2g43010; MFL8.13; Transcription factor PIF4; Basic helix-loop-helix protein 9; AtbHLH9; bHLH 9; Phytochrome-interacting factor 4; Short under red-light 2; Transcription factor EN 102; bHLH transcription factor bHLH009
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-430
  • 氨基酸序列
    MEHQGWSFEE NYSLSTNRRS IRPQDELVEL LWRDGQVVLQ SQTHREQTQT QKQDHHEEAL RSSTFLEDQE TVSWIQYPPD EDPFEPDDFS SHFFSTMDPL QRPTSETVKP KSSPEPPQVM VKPKACPDPP PQVMPPPKFR LTNSSSGIRE TEMEQYSVTT VGPSHCGSNP SQNDLDVSMS HDRSKNIEEK LNPNASSSSG GSSGCSFGKD IKEMASGRCI TTDRKRKRIN HTDESVSLSD AIGNKSNQRS GSNRRSRAAE VHNLSERRRR DRINERMKAL QELIPHCSKT DKASILDEAI DYLKSLQLQL QVMWMGSGMA AAAASAPMMF PGVQPQQFIR QIQSPVQLPR FPVMDQSAIQ NNPGLVCQNP VQNQIISDRF ARYIGGFPHM QAATQMQPME MLRFSSPAGQ QSQQPSSVPT KTTDGSRLDH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor acting negatively in the phytochrome B signaling pathway. May regulate the expression of a subset of genes involved in cell expansion by binding to the G-box motif. Activated by CRY1 and CRY2 in response to low blue light (LBL) by direct binding at chromatin on E-box variant 5'-CA[CT]GTG-3' to stimulate specific gene expression to adapt global physiology (e.g. hypocotyl elongation in low blue light).
  • 基因功能参考文献：
    1. The interaction between TOC1 and PIF4 mediates the circadian gating of thermoresponsive growth. PMID: 27966533
    2. DCL1 interacts with PIF4.PIF4 acts as a transcription factor for some miRNA genes and is necessary for the proper accumulation of miRNAs. PMID: 29522510
    3. Study shows that PHYTOCHROME INTERACTING FACTOR 4 (PIF4)-mediated thermosensory growth and architecture adaptations are directly linked to suppression of immunity at elevated temperature. PIF4 positively regulates growth and development and negatively regulates immunity. PMID: 28041792
    4. Results show that the phytochrome-interacting factor 4 (PIF4) is required for cytokinin (CK) upregulation of the transcription of CKRC2/YUC8 and that the transcription of PIF4 itself is induced by CK via the AHKs-ARR1/12 signaling pathway. PMID: 27827441
    5. Analysis of transcriptomes indicated that increased expression of the transcription factor PHYTOCHROME-INTERACTING FACTOR (PIF4) may contribute to hybrid vigor by targeting the auxin biosynthesis gene YUCCA8 and the auxin signaling gene IAA29. PMID: 28396418
    6. BAF60 binds nucleosome-free regions of multiple G box-containing genes, opposing in cis the promoting effect of the photomorphogenic and thermomorphogenic regulator Phytochrome Interacting Factor 4 (PIF4) on hypocotyl elongation. Furthermore, BAF60 expression level is regulated in response to light and daily rhythms. PMID: 28619072
    7. COG1 binds to the promoter regions of PIF4 and PIF5, and PIF4 and PIF5 bind to the promoter regions of key Brassinosteroid biosynthetic genes, such as DWF4 and BR6ox2, to directly promote their expression. PMID: 28438793
    8. These findings reveal a mechanistic framework in which DET1/COP1 and HY5 regulatory modules act independently to regulate growth through the environmental signal integrator PIF4. PMID: 28076780
    9. ethylene-insensitive 3 (EIN3) is involved into the direct regulation of YUC9 transcription in this process. Furthermore, we demonstrated that PHYTOCHROME INTERACTING FACTOR4 (PIF4) functions as a transcriptional activator for YUC5/8/9. PMID: 27716807
    10. this review discusses PIF4 as a key integrator of multiple signaling pathways, which optimizes growth in the environment PMID: 27432188
    11. LEC1 acts as a coactivator of PIFs in transcriptional regulation during postembryonic growth. PMID: 26566918
    12. For growth under a canopy, where blue light is diminished, CRY1 and CRY2 perceive this change and respond by directly contacting two bHLH transcription factors, PIF4 and PIF5. PMID: 26724867
    13. CRY1 represses auxin biosynthesis in response to elevated temperature through PIF4. PMID: 26699514
    14. PIF4 and PIF5 negatively regulate red light-induced anthocyanin accumulation through transcriptional repression of the anthocyanin biosynthetic genes in Arabidopsis. PMID: 26259175
    15. Phytochrome-interacting transcription factors PIF4 and PIF5 induce leaf senescence in Arabidopsis. PMID: 25119965
    16. The expression level of PIF3, 4, and 5 was significantly up-regulated during both age-triggered and dark-induced leaf senescence. PMID: 25296857
    17. At least two downstream modules participate in diurnal rhythmic hypocotyl growth: PIF4 and/or PIF5 modulation of auxin-related pathways and PIF-independent regulation of the gibberellic acid (GA) pathway. PMID: 21430186
    18. The PIF4 and PIF5 transcription factors promote flowering by at least two means: inducing FT expression in warm night and acting outside of FT by an unknown mechanism in warm days. PMID: 24574484
    19. the light-regulated PIF4 (phytochrome-interacting factor 4) factor is a phosphorylation target of the BR signaling kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2), which marks this transcriptional regulator for proteasome degradation PMID: 25085420
    20. circadian clock and PIF4/PIF5 mediated external coincidence mechanism in transcription of ST2A PMID: 24317064
    21. Data indicate that PIF4 and PIF5 negatively regulate auxin signaling. that PIF4 and PIF5 negatively modulate auxin-mediated phototropism through directly activating IAA19 and IAA29, which physically interact with auxin factor7 (ARF7). PMID: 23757399
    22. PIF4 and/or PIF5 act as modulators of auxin signaling implicated in the rhythmic elongation of hypocotyls. PMID: 23037003
    23. Circadian clock affects the expression of PIF4 in response to high temperature. PMID: 23037004
    24. regulates elongation growth by controlling directly the expression of genes that code for auxin biosynthesis and auxin signaling components PMID: 22536829
    25. PhyB-mediated, post-translational regulation allows PIF3 accumulation to peak just before dawn, at which time it accelerates hypocotyl growth, together with PIF4 and PIF5, by directly regulating the induction of growth-related genes. PMID: 22409654
    26. BZR1 and PIF4 interact with each other in vitro and in vivo, bind to nearly 2,000 common target genes, and synergistically regulate many of these target genes PMID: 22820378
    27. our results illuminate a molecular framework by which the PIF4 transcriptional regulator integrates its action into the auxin pathway through activating the expression of specific auxin biosynthetic gene (YUCCA8). PMID: 22479194
    28. demonstration of a direct mechanism by which increasing temperature causes the bHLH transcription factor PHYTOCHROME INTERACTING FACTOR4 (PIF4) to activate FLOWERING LOCUS T (FT) PMID: 22437497
    29. These results demonstrate direct molecular links among PIF4, auxin, and elongation growth at high temperature. PMID: 22123947
    30. an external coincidence model involving the clock-controlled PIF4/PIF5-ATHB2 pathway is crucial for the diurnal and photoperiodic control of plant growth in A. thaliana. PMID: 21666227
    31. PIF4 and PIF5 responsible not only for red light signaling through the phytochromes but also for blue light signaling in the photomorphogenic control of hypocotyl elongation PMID: 21150090
    32. A non-synonymous coding SNP at PIF4 is associated significantly with variation in average early internode length and marginally associated with total inflorescence length. PMID: 20456226
    33. microarray analysis shows that PIF4 and PIF5 are part of an inhibitory mechanism that represses the expression of some light-responsive genes in the dark, and that they are also needed for full expression of several growth-related genes in the light. PMID: 19619162
    34. Overexpression of SHORT HYPOCOTYL UNDER BLUE1 enhanced the expression of PHYTOCHROME-INTERACTING FACTOR4 (PIF4) under red light. PMID: 16500988
    35. Data provide evidence that the mechanism by which PIF4 operate on the phyB signaling pathway under prolonged red light is through maintaining low phyB protein levels, in an additive or synergistic manner. PMID: 18252845
    36. PIF4 Acts in a phytochrome-Dependent Manner to Mediate Changes in Stomatal Index. PMID: 19185498
    37. Results suggest that PIF4 is an important component of plant high temperature signaling and integrates multiple environmental cues during plant development. PMID: 19249207

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BHLH protein family
  • 数据库链接：

    KEGG: ath:AT2G43010

    STRING: 3702.AT2G43010.1

    UniGene: At.19015