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  4. Recombinant Arabidopsis thaliana Transcription factor ICE1 (SCRM)

Recombinant Arabidopsis thaliana Transcription factor ICE1 (SCRM)

  • 中文名称：
    拟南芥SCRM重组蛋白
  • 货号：
    CSB-YP881796DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥SCRM重组蛋白
  • 货号：
    CSB-EP881796DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥SCRM重组蛋白
  • 货号：
    CSB-EP881796DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥SCRM重组蛋白
  • 货号：
    CSB-BP881796DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥SCRM重组蛋白
  • 货号：
    CSB-MP881796DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SCRM
  • Uniprot No.：
  • 别名：
    SCRM; BHLH116; EN45; ICE1; At3g26744; MDJ14.1; MLJ15.14Transcription factor ICE1; Basic helix-loop-helix protein 116; AtbHLH116; bHLH 116; Inducer of CBF expression 1; Transcription factor EN 45; Transcription factor SCREAM; bHLH transcription factor bHLH116
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-494
  • 氨基酸序列
    MGLDGNNGGG VWLNGGGGER EENEEGSWGR NQEDGSSQFK PMLEGDWFSS NQPHPQDLQM LQNQPDFRYF GGFPFNPNDN LLLQHSIDSS SSCSPSQAFS LDPSQQNQFL STNNNKGCLL NVPSSANPFD NAFEFGSESG FLNQIHAPIS MGFGSLTQLG NRDLSSVPDF LSARSLLAPE SNNNNTMLCG GFTAPLELEG FGSPANGGFV GNRAKVLKPL EVLASSGAQP TLFQKRAAMR QSSGSKMGNS ESSGMRRFSD DGDMDETGIE VSGLNYESDE INESGKAAES VQIGGGGKGK KKGMPAKNLM AERRRRKKLN DRLYMLRSVV PKISKMDRAS ILGDAIDYLK ELLQRINDLH NELESTPPGS LPPTSSSFHP LTPTPQTLSC RVKEELCPSS LPSPKGQQAR VEVRLREGRA VNIHMFCGRR PGLLLATMKA LDNLGLDVQQ AVISCFNGFA LDVFRAEQCQ EGQEILPDQI KAVLFDTAGY AGMI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that regulates the cold-induced transcription of CBF/DREB1 genes. Binds specifically to the MYC recognition sites (5'-CANNTG-3') found in the CBF3/DREB1A promoter. Mediates stomatal differentiation in the epidermis probably by controlling successive roles of SPCH, MUTE, and FAMA. Functions as a dimer with SPCH during stomatal initiation.
  • 基因功能参考文献：
    1. MPK3 and MPK6 can phosphorylate ICE1, a basic-helix-loop-helix transcription factor that regulates the expression of CBF genes PMID: 29056551
    2. unified ICE-CBF pathway provides transcriptional feedback control of freezing tolerance during cold acclimation PMID: 26311645
    3. Seedling growth was severely reduced in a T-DNA insertion mutant of ICE1, ice1-2, when grown on 1/2 MS medium lacking sugars, but was restored to wild-type (WT) levels by supplementation with 56 mM glucose. PMID: 26048037
    4. ICE2 gene has originated from a duplication event about 17.9MYA followed by sub- and neofunctionalization of the ancestral ICE1 gene. PMID: 25443829
    5. uncover the unexpected roles of OST1 in modulating C-repeat-binding factor-dependent cold signaling in Arabidopsis PMID: 25669882
    6. propose that the ZHOUPI/ICE1 complex might have ancient origins, acquiring novel megagametophyte-specific functions in heterosporous land plants that were conserved in the angiosperm endosperm PMID: 24553285
    7. Jasmonate functions as a critical upstream signal of the ICE-CBF/DREB1 pathway to positively regulate Arabidopsis freezing tolerance. PMID: 23933884
    8. The serine 403 to alanine substitution increases the transactivation activity of ICE1 and the freezing tolerance of Arabidopsis. PMID: 21447070
    9. The inducer of CBF expression1 (ICE1) protein that is involved in transcriptional control of cold responses is found to bind to a MYC element in this BAP1 promoter and is required for the cooling induction of BAP1. PMID: 21098676
    10. Regulon genes repressed by siz1 did not affect expression of ICE1, which encodes a MYC transcription factor. PMID: 17416732

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Widely expressed in the whole plant with high expression in leaves and stem. Broad expression within stomatal cell lineages of leaf epidermis.
  • 数据库链接：

    KEGG: ath:AT3G26744

    STRING: 3702.AT3G26744.1

    UniGene: At.25302