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  4. Recombinant Arabidopsis thaliana Transcription factor E2FA (E2FA)

Recombinant Arabidopsis thaliana Transcription factor E2FA (E2FA)

  • 中文名称：
    拟南芥E2FA重组蛋白
  • 货号：
    CSB-YP872305DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥E2FA重组蛋白
  • 货号：
    CSB-EP872305DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥E2FA重组蛋白
  • 货号：
    CSB-EP872305DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥E2FA重组蛋白
  • 货号：
    CSB-BP872305DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥E2FA重组蛋白
  • 货号：
    CSB-MP872305DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    E2FA
  • Uniprot No.：
  • 别名：
    E2FA; E2F3; E2F4; At2g36010; F11F19.8Transcription factor E2FA; E2F transcription factor-3; AtE2F3
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-485
  • 氨基酸序列
    MSGVVRSSPG SSQPPPPPPH HPPSSPVPVT STPVIPPIRR HLAFASTKPP FHPSDDYHRF NPSSLSNNND RSFVHGCGVV DREEDAVVVR SPSRKRKATM DMVVAPSNNG FTSSGFTNIP SSPCQTPRKG GRVNIKSKAK GNKSTPQTPI STNAGSPITL TPSGSCRYDS SLGLLTKKFV NLIKQAKDGM LDLNKAAETL EVQKRRIYDI TNVLEGIDLI EKPFKNRILW KGVDACPGDE DADVSVLQLQ AEIENLALEE QALDNQIRQT EERLRDLSEN EKNQKWLFVT EEDIKSLPGF QNQTLIAVKA PHGTTLEVPD PDEAADHPQR RYRIILRSTM GPIDVYLVSE FEGKFEDTNG SGAAPPACLP IASSSGSTGH HDIEALTVDN PETAIVSHDH PHPQPGDTSD LNYLQEQVGG MLKITPSDVE NDESDYWLLS NAEISMTDIW KTDSGIDWDY GIADVSTPPP GMGEIAPTAV DSTPR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The binding of retinoblastoma-related proteins represses transactivation. Regulates gene expression both positively and negatively. Activates the expression of E2FB. Involved in the control of cell-cycle progression from G1 to S phase. Stimulates cell proliferation and delays differentiation.
  • 基因功能参考文献：
    1. The E2Fa transcription factor of Arabidopsis thaliana is an essential component that regulates the asymmetric cell division marking lateral root initiation. PMID: 22003076
    2. AtE2Fa and AtE2Fb have specific expression patterns and may play similar but distinct roles during cell cycle progression. PMID: 16514015
    3. Overlap between CYCD3;1 and E2Fa modulated genes substantiates their action in a common pathway with a key role in controlling the G1/S transition, with additional targets for E2Fa in cell wall biogenesis and development. PMID: 19662336
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Interaction with DPA induces an exclusive nuclear localization, but an interaction with DPB has no effect.
  • 蛋白家族：
    E2F/DP family
  • 组织特异性：
    Highly expressed in the shoot apical meristem, emerging leaf primordia, and vascular tissues of young leaf primordia. Expressed in flowers, in epidermis and cortex of hypocotyls, and at lower levels in leaves.
  • 数据库链接：

    KEGG: ath:AT2G36010

    UniGene: At.10190