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  4. Recombinant Arabidopsis thaliana Serine/threonine-protein kinase HT1 (HT1)

Recombinant Arabidopsis thaliana Serine/threonine-protein kinase HT1 (HT1)

  • 中文名称：
    拟南芥HT1重组蛋白
  • 货号：
    CSB-YP646662DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥HT1重组蛋白
  • 货号：
    CSB-EP646662DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥HT1重组蛋白
  • 货号：
    CSB-EP646662DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥HT1重组蛋白
  • 货号：
    CSB-BP646662DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥HT1重组蛋白
  • 货号：
    CSB-MP646662DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HT1
  • Uniprot No.：
  • 别名：
    HT1; SUU; At1g62400; F24O1.13; Serine/threonine/tyrosine-protein kinase HT1; EC 2.7.10.1; EC 2.7.11.1; High leaf temperature protein 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-390
  • 氨基酸序列
    MSGLCFNPFR LRWSLRSKLP LEPSLPNLPC NPSSSKTNRY AEAETMEKKR FDSMESWSMI LESENVETWE ASKGEREEWT ADLSQLFIGN KFASGAHSRI YRGIYKQRAV AVKMVRIPTH KEETRAKLEQ QFKSEVALLS RLFHPNIVQF IAACKKPPVY CIITEYMSQG NLRMYLNKKE PYSLSIETVL RLALDISRGM EYLHSQGVIH RDLKSNNLLL NDEMRVKVAD FGTSCLETQC REAKGNMGTY RWMAPEMIKE KPYTRKVDVY SFGIVLWELT TALLPFQGMT PVQAAFAVAE KNERPPLPAS CQPALAHLIK RCWSENPSKR PDFSNIVAVL EKYDECVKEG LPLTSHASLT KTKKAILDHL KGCVTSISSP FSSSSVPVNA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine/tyrosine kinase involved in the control of stomatal movement in response to CO(2). Functions as a major negative regulator of CO(2)-induced stomatal closing. Does not seem to be involved in stomatal closure in response to abscisic acid (ABA) or light. Involved in the control of red light-induced stomatal opening. Is epistatic to SRK2E/OST1 function during stomatal responses to red light and altered CO(2). Phosphorylates SRK2E/OST1 and GHR1 to prevents SRK2E/OST1- and GHR1-induced activation of SLAC1, thus preventing stomatal closure.
  • 基因功能参考文献：
    1. Both the loss-of-function and gain-of-function ht1 mutants have completely disrupted CO2 responses, although they have normal responses to ABA. Light-induced stomatal opening is smaller in ht1-3 allele and much smaller in ht1-2. The results indicate that HT1 is a critical regulator for CO2 signaling and is partially involved in the light-induced stomatal opening pathway. PMID: 27034327
    2. These results demonstrate that HT1 is essential for red light-induced stomatal opening and interacts genetically with OST1 during stomatal responses to red light and altered [CO2 ]. PMID: 26192339
  • 亚细胞定位：
    Cell membrane.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Mainly localizes in guard cells. Expressed at low level in leaves, stems, roots and flowers.
  • 数据库链接：

    KEGG: ath:AT1G62400

    STRING: 3702.AT1G62400.1

    UniGene: At.28069