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  4. Recombinant Arabidopsis thaliana Ras-related protein RABF2b (RABF2B)

Recombinant Arabidopsis thaliana Ras-related protein RABF2b (RABF2B)

  • 中文名称：
    拟南芥RABF2B重组蛋白
  • 货号：
    CSB-YP871121DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥RABF2B重组蛋白
  • 货号：
    CSB-EP871121DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥RABF2B重组蛋白
  • 货号：
    CSB-EP871121DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥RABF2B重组蛋白
  • 货号：
    CSB-BP871121DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥RABF2B重组蛋白
  • 货号：
    CSB-MP871121DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RABF2B
  • Uniprot No.：
  • 别名：
    RABF2B; ARA-7; RAB5B; At4g19640; F24J7.190; Ras-related protein RABF2b; AtRABF2b; Ras-related protein Ara-7; Ras-related protein Rab5B; AtRab5B
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-200
  • 氨基酸序列
    MAAAGNKSIN AKLVLLGDVG AGKSSLVLRF VKDQFVEFQE STIGAAFFSQ TLAVNDATVK FEIWDTAGQE RYHSLAPMYY RGAAAAIIVF DVTNQASFER AKKWVQELQA QGNPNMVMAL AGNKSDLLDA RKVTAEDAQT YAQENGLFFM ETSAKTATNV KEIFYEIARR LPRVQPTENP TGMVLPDRAM DRAVSSSCCA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endosomal protein that may be involved in endocytosis. Involved in the trafficking of proteins from prevacuolar compartments (PVCs) to vacuoles. May activate the MON1-CCZ1 complex which acts as guanine nucleotide exchange factors (GEF) for Rab7 protein family, and serves as a link between Rab5 and Rab7 families in PVCs, and mediates PVC maturation. Involved in vacuolar transport of storage proteins with EREX as effector. Regulates membrane trafficking to protein storage vacuoles (PSVs).
  • 基因功能参考文献：
    1. VHA-a3 targets to the tonoplast through a Rab5-mediated but Rab7-independent pathway, shedding new lights on the unconventional vacuolar trafficking route in plant cells.[Rab5] PMID: 28198146
    2. Ara7 was localized to the the extrahaustorial membrane during powdery mildew infection. PMID: 27318282
    3. A loss of FAB1 function causes the release of late endosomal proteins, Ara7, and SNX1 from the endosome membrane, indicating that FAB1, or its product PtdIns(3,5)P2, mediates the maturation process of the late endosomes. PMID: 26353760
    4. ARA7(Q69L) overexpression induces the formation of enlarged multivesicular bodies. PMID: 23682115
    5. The crystal structure of ARA7-GDP-divalent calcium ion (Ca2+)-VPS9a reveals that the metal ion directly interacts with the aspartate finger of VPS9a. PMID: 23519409

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  • 亚细胞定位：
    Early endosome membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Prevacuolar compartment membrane; Lipid-anchor. Endosome, multivesicular body membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Cytoplasm.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 组织特异性：
    Expressed in roots and actively dividing cells.
  • 数据库链接：

    KEGG: ath:AT4G19640

    STRING: 3702.AT4G19640.1

    UniGene: At.2030