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  4. Recombinant Arabidopsis thaliana Protein mago nashi homolog (At1g02140)

Recombinant Arabidopsis thaliana Protein mago nashi homolog (At1g02140)

  • 中文名称：
    拟南芥MAGO重组蛋白
  • 货号：
    CSB-YP013364DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥MAGO重组蛋白
  • 货号：
    CSB-EP013364DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥MAGO重组蛋白
  • 货号：
    CSB-EP013364DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥MAGO重组蛋白
  • 货号：
    CSB-BP013364DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥MAGO重组蛋白
  • 货号：
    CSB-MP013364DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MAGO
  • Uniprot No.：
  • 别名：
    MAGO; At1g02140; T7I23.7Protein mago nashi homolog; AtMAGO
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-150
  • 氨基酸序列
    MAAEEATEFY LRYYVGHKGK FGHEFLEFEF REDGKLRYAN NSNYKNDTII RKEVFLTPAV LKECKRIVSE SEILKEDDNN WPEPDRVGKQ ELEIVLGNEH ISFATSKIGS LVDCQSSNDP EGLRIFYYLV QDLKCLVFSL ISLHFKIKPI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGO-Y14 heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGO-Y14 heterodimer interacts with the EJC key regulator PYM leading to EJC disassembly in the cytoplasm. Can increase in vitro the expression from reporter constructs that contain leader introns required for the expression of different genes. In association with MAGO and PYM, participates in intron-mediated enhancement of gene expression. The MAGO-Y14 heterodimer works synergistically with the NMD pathway to regulate male gametophyte development.
  • 基因功能参考文献：
    1. the C terminal domain (CTD) of AtMago is required for the AtMago-AtY14 heterodimerization during exon-exon junction complex assembly PMID: 26867216
    2. Overexpression of Mago and Y14 in plants produces an increase in PYM protein levels. PMID: 21676911
  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus speckle. Nucleus. Cytoplasm, P-body. Cytoplasm.
  • 蛋白家族：
    Mago nashi family
  • 组织特异性：
    Expressed in root and shoot meristems, cotyledons, vascular tissues of leaves, receptacle of flowers and siliques, and pollen grains.
  • 数据库链接：

    KEGG: ath:AT1G02140

    STRING: 3702.AT1G02140.1

    UniGene: At.21578