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  4. Recombinant Arabidopsis thaliana Protein WUSCHEL (WUS)

Recombinant Arabidopsis thaliana Protein WUSCHEL (WUS)

  • 中文名称：
    拟南芥WUS重组蛋白
  • 货号：
    CSB-YP882815DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥WUS重组蛋白
  • 货号：
    CSB-EP882815DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥WUS重组蛋白
  • 货号：
    CSB-EP882815DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥WUS重组蛋白
  • 货号：
    CSB-BP882815DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥WUS重组蛋白
  • 货号：
    CSB-MP882815DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WUS
  • Uniprot No.：
  • 别名：
    WUS; PGA6; At2g17950; T27K22.18; Protein WUSCHEL; AtWUS; Plant growth activator 6
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-292
  • 氨基酸序列
    MEPPQHQHHH HQADQESGNN NNNKSGSGGY TCRQTSTRWT PTTEQIKILK ELYYNNAIRS PTADQIQKIT ARLRQFGKIE GKNVFYWFQN HKARERQKKR FNGTNMTTPS SSPNSVMMAA NDHYHPLLHH HHGVPMQRPA NSVNVKLNQD HHLYHHNKPY PSFNNGNLNH ASSGTECGVV NASNGYMSSH VYGSMEQDCS MNYNNVGGGW ANMDHHYSSA PYNFFDRAKP LFGLEGHQEE EECGGDAYLE HRRTLPLFPM HGEDHINGGS GAIWKYGQSE VRPCASLELR LN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that plays a central role during early embryogenesis, oogenesis and flowering, probably by regulating expression of specific genes. Required to specify stem cell identity in meristems, such as shoot apical meristem (SAM). May induce shoot stem cells activity in order to maintain the stem cell identity. Involved in the developmental root meristem. In shoot apices, it is sufficient to induce the expression of CLV3, a putative ligand of the CLV signaling pathway. Also required to sustain organogenesis in the floral meristem by contributing to the expression of its own repressor, the AGAMOUS (AG) gene at the end of flower development. Binds directly to the 5'-TTAAT[GC][GC]-3' DNA sequence in the regulatory sequence of AG and activates its expression directly. Regulates one important step in ovule development to induce integument formation from the underlying chalazal domain. Participates in the promotion of vegetative to embryonic transition. Required to repress LEC1 expression.
  • 基因功能参考文献：
    1. Study find that WUSCHEL, a key gene required for apical meristem maintenance, is a cytokinin-dependent type-B ARABIDOPSIS RESPONSE REGULATORS target gene, demonstrating the importance of the cytokinin transcription factor network in shoot development. PMID: 29686312
    2. Cytokinin signaling in the rib meristem acts through the transcriptional regulatory domains, the acidic domain and the WUSCHEL-box, to stabilize the WUS protein. PMID: 29659567
    3. ARR12 binds to the promoter of WUS. PMID: 28681564
    4. WUS is regulated by a balance between positive regulation by cytokinin and negative regulation by CLAVATA protein. PMID: 27208247
    5. Dynamic expression reveals a two-step patterning of WUS and CLV3 during axillary shoot meristem formation in Arabidopsis PMID: 28399422
    6. results provide important insights into the molecular framework for cytokinin-directed shoot regeneration and reveal a two-step mechanism for de novo activation of WUS. PMID: 28389585
    7. there is a positive feedback regulation between WUS and OBE3 that contributes to shoot meristem homeostasis PMID: 27196372
    8. The interplay between cytokinin signaling, WUS/CLV feedback loop and boundary signals can account for positioning of the WUS expression in the process of Arabidopsis thaliana shoot apical meristem patterning. PMID: 26872130
    9. The branching ovules of the mutant resemble those of some fossil gymnosperms, implicating BEL1 and HD-ZIPIII genes as players in the evolution of the unbranched ovule form in extant angiosperms. PMID: 26700684
    10. The stem cell niche in Arabidopsis copes with environmental hazards by enhancing the IRES-dependent translation of WUS mRNA under the control of the AtLa1 protein. PMID: 25764476
    11. stem cell specification pathway is normally repressed by the activity of the HD-zip III transcription factors PHABULOSA (PHB), PHAVOLUTA (PHV) and CORONA (CNA). PMID: 26011610
    12. In the floral meristem, the binding of AG to WUS is reduced in top1a-2, which results in reduced H3K27me3 levels at WUS and prolonged WUS expression, and consequently loss of floral determinacy. PMID: 25070639
    13. Data indicate that the stem cell inducing transcription factor WUSCHEL (WUS) moves to the stem cells via plasmodesmata in a highly regulated fashion and that this movement is required for WUS function. PMID: 25246576
    14. AtWus may therefore increase the differentiation potential of cotton callus by triggering the auxin transport and signaling pathways. PMID: 24498119
    15. GRP23 expression can be activated by WUS. PMID: 24086632
    16. HECATE1 (HEC1) is a target of WUS and that it contributes to SAM function by promoting stem cell proliferation, while antagonizing niche cell activity. HEC1 represses the stem cell regulators WUS and CLAVATA3 (CLV3) PMID: 24576426
    17. Strong upregulation of WUS in the inflorescence meristem leads to ectopic expression of the AGAMOUS homeotic gene to a level that switches cell fate from floral meristem founder cell to carpel founder cell. PMID: 24496620
    18. Results suggest that ASL11/LBD15 affects cellular differentiation in the SAM and regulates WUS expression. PMID: 23397191
    19. Cytokinin treatment induces the expression of the shoot meristematic gene WUSCHEL (WUS) in converting LRP (cLRP) within 24-30 h, and WUS is required for lateral root primorida(LRP)--> New shoot meristems (SMs) conversion. PMID: 23181633
    20. AG directly represses WUS expression by binding to the WUS locus and recruiting, directly or indirectly, PcG that methylates histone H3 Lys-27 at WUS. PMID: 22028461
    21. DNA methylation and histone modifications regulate de novo shoot regeneration by modulating WUS expression and auxin signaling. PMID: 21876682
    22. Studies indicate that the shoot and root meristems are promoted by WUSCHEL (WUS) and WOX5. PMID: 22076631
    23. the WUS protein, after being synthesized in cells of the organizing center, migrates into the central zone, where it activates CLV3 transcription by binding to its promoter elements PMID: 21979915
    24. WUSCHEL-mediated cellular feedback network imparts robustness to stem cell homeostasis. PMID: 21406977
    25. WUS produces severe phenotypes by disrupting the development of somatic embryos on the maturation medium and inhibiting germination. PMID: 20424847
    26. New function for WUS in mediating the balance between differentiation and non-differentiating cells of the peripheral zone. PMID: 20876644
    27. WUS expression is controlled by the ratio of cytokinin with auxin. PMID: 20514542
    28. CLAVATA1 is part of a negative feedback regulation of WUSCHEL protein in Arabidopsis thaliana. PMID: 20493817
    29. Our results demonstrate that WUS acts mainly as a repressor and that its function changes from that of a repressor to that of an activator in the case of regulation of the expression of AG. PMID: 19897670
    30. Ultrapetal1 negatively regulates WUSCHEL to establish floral meristem determinacy. PMID: 15342527
    31. results suggest that overexpression of WUS could trigger cell pluripotence and reestablish new meristems from differentiated tissues PMID: 15952065
    32. The homeobox gene WUSCHEL (WUS) is expressed in the organizing center underneath the stem cells and integrates regulatory information from several pathways to define the boundaries of the stem cell niche. PMID: 15980263
    33. data indicate that ARR genes might negatively influence meristem size and that their repression by WUS might be necessary for proper meristem function PMID: 16372013
    34. Detailed characterization of a semidominant roa allele revealed an essential role for the conserved C-terminal domain that mediates an interaction between WUS and two members of a small family of corepressor-like proteins in Arabidopsis. PMID: 16461579
    35. facilitates high cytokinin activity in the shoot apical meristem PMID: 16877025
    36. WUS expression is initiated very early during anther development in the precursor cells of the stomium and terminates just before the stomium cells enter terminal differentiation PMID: 17027956
    37. Importantly, both WOX5 and WUS maintain stem cells in either a root or shoot context. PMID: 17429400
    38. Data suggest that, by limiting WUS expression to the organizing center, BARD1 regulates shoot apical meristem organization and maintenance. PMID: 18591352
    39. WUS-dependent organising centre signalling to the stem cells is promoted by AGO1 and subsequently maintained by a provascular ZLL-dependent signalling pathway. PMID: 18653559
    40. Establishment of auxin gradients and PIN1-mediated polar auxin transport are essential for WUS induction and somatic embryogenesis. PMID: 19453451
    41. Data show that cytokinin-induced WUSCHEL expression occurs through both CLAVATA-dependent and CLAVATA-independent pathways. PMID: 19717465

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WUS homeobox family
  • 组织特异性：
    In the active shoot meristem, it is specifically expressed in a small cell group underneath the presume position of stem cells. Also expressed in the floral meristem. Expressed in the nucellus of ovule primordia.
  • 数据库链接：

    KEGG: ath:AT2G17950

    STRING: 3702.AT2G17950.1

    UniGene: At.51