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  4. Recombinant Arabidopsis thaliana Protein EPIDERMAL PATTERNING FACTOR 2 (EPF2)

Recombinant Arabidopsis thaliana Protein EPIDERMAL PATTERNING FACTOR 2 (EPF2)

  • 中文名称：
    拟南芥EPF2重组蛋白
  • 货号：
    CSB-YP818527DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥EPF2重组蛋白
  • 货号：
    CSB-EP818527DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥EPF2重组蛋白
  • 货号：
    CSB-EP818527DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥EPF2重组蛋白
  • 货号：
    CSB-BP818527DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥EPF2重组蛋白
  • 货号：
    CSB-MP818527DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EPF2
  • Uniprot No.：
  • 别名：
    EPF2; At1g34245; F23M19Protein EPIDERMAL PATTERNING FACTOR 2 [Cleaved into: MEPF2]
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-120
  • 氨基酸序列
    IRTPP LKNTVNGGEK KNADIEQAQT HHKKEISKNG GVEMEMYPTG SSLPDCSYAC GACSPCKRVM ISFECSVAES CSVIYRCTCR GRYYHVPSRA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Controls stomatal patterning. Regulates the number of cells that enter, and remain in, the stomatal lineage by inhibiting protodermal cells from adopting the meristemoid mother cell (MMC) fate in a non-cell-autonomous manner. Mediates stomatal development inhibition. MEPF2: mobile signal controlling stomatal development in a non-cell-autonomous manner. Uses ERECTA as major receptor. Inactivated by cleavage by CRSP (AC Q9LNU1). May act by competing with somatogen (AC Q9SV72) for the same receptor, TMM (AC Q9SSD1).
  • 基因功能参考文献：
    1. In a ROS1 mutant, the promoter region of the peptide ligand gene EPF2 is hypermethylated, which reduces EPF2 expression and leads to overproduction of stomatal lineage cells. PMID: 24898766
    2. the secreted protease CRSP, which cleaves the pro-peptide EPF2, in turn repressing stomatal development PMID: 25043023
    3. Calculation of the number of stomata per leaf and leaf size showed that plants lacking EPF2 and EPFL9 had a higher proportion of non-stomatal epidermal cells. PMID: 22232766
    4. Results suggest that EPF2 is expressed earlier in leaf development than EPF1 and is involved in determining the number of cells that enter, and remain in, the stomatal lineage. [EPF2] PMID: 19398336
    5. EPF2 is a small secretory peptide expressed in meristemoid mother cells and regulates epidermal cell density. [EPF2] PMID: 19435754

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Plant cysteine rich small secretory peptide family, Epidermal patterning factor subfamily
  • 组织特异性：
    Expressed in leaves, especially by the MMCs and their early descendants cells (stomatal lineage cells) including guard mother cells (GMCs).
  • 数据库链接：

    KEGG: ath:AT1G34245

    STRING: 3702.AT1G34245.1

    UniGene: At.39780