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  4. Recombinant Arabidopsis thaliana Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE)

Recombinant Arabidopsis thaliana Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM (FIE)

  • 中文名称：
    拟南芥FIE重组蛋白
  • 货号：
    CSB-YP862881DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥FIE重组蛋白
  • 货号：
    CSB-EP862881DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥FIE重组蛋白
  • 货号：
    CSB-EP862881DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥FIE重组蛋白
  • 货号：
    CSB-BP862881DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥FIE重组蛋白
  • 货号：
    CSB-MP862881DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FIE
  • Uniprot No.：
  • 别名：
    FIE; FIS3; At3g20740; MOE17.5; Polycomb group protein FERTILIZATION-INDEPENDENT ENDOSPERM; Protein FERTILIZATION-INDEPENDENT SEED 3
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-369
  • 氨基酸序列
    MSKITLGNES IVGSLTPSNK KSYKVTNRIQ EGKKPLYAVV FNFLDARFFD VFVTAGGNRI TLYNCLGDGA ISALQSYADE DKEESFYTVS WACGVNGNPY VAAGGVKGII RVIDVNSETI HKSLVGHGDS VNEIRTQPLK PQLVITASKD ESVRLWNVET GICILIFAGA GGHRYEVLSV DFHPSDIYRF ASCGMDTTIK IWSMKEFWTY VEKSFTWTDD PSKFPTKFVQ FPVFTASIHT NYVDCNRWFG DFILSKSVDN EILLWEPQLK ENSPGEGASD VLLRYPVPMC DIWFIKFSCD LHLSSVAIGN QEGKVYVWDL KSCPPVLITK LSHNQSKSVI RQTAMSVDGS TILACCEDGT IWRWDVITK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Required to prevent the proliferation of the central cell by repressing unknown target genes before fertilization. Probably also involved in floral repression mechanism established during early plant development. Regulates the anteroposterior organization of the endosperm. Interacts with the promoter and represses the transcription of genes such as PHE1, that are paternally active and maternally silenced.
  • 基因功能参考文献：
    1. Arabidopsis FIE binding sites tend to be in gene coding regions and co-localize with H3K27me3. PMID: 24001316
    2. H3K27me3 deposition is abolished genome-wide in fertilization independent endosperm protein mutants demonstrating the essential function of polycomb repressive complex 2 PMID: 21423668
    3. Used to demonstrate the feasibility of bimolecular fluorescence complementation in plants. PMID: 15469499
  • 亚细胞定位：
    Nucleus. Note=Excluded from the nucleolus.
  • 蛋白家族：
    WD repeat ESC family
  • 组织特异性：
    Expressed in cauline leaves, root and stems. In the male reproductive organ, it is expressed in the developing anther; and is abundant in microspore mother cells, in microsporocytes and in the tapetum, but is absent from vascular bundles, the connective t
  • 数据库链接：

    KEGG: ath:AT3G20740

    STRING: 3702.AT3G20740.1

    UniGene: At.20884