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  4. Recombinant Arabidopsis thaliana Polyamine oxidase 3 (PAO3)

Recombinant Arabidopsis thaliana Polyamine oxidase 3 (PAO3)

  • 中文名称：
    拟南芥PAO3重组蛋白
  • 货号：
    CSB-YP864894DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PAO3重组蛋白
  • 货号：
    CSB-EP864894DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PAO3重组蛋白
  • 货号：
    CSB-EP864894DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PAO3重组蛋白
  • 货号：
    CSB-BP864894DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PAO3重组蛋白
  • 货号：
    CSB-MP864894DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PAO3
  • Uniprot No.：
  • 别名：
    PAO3; At3g59050; F17J16.100; Polyamine oxidase 3; AtPAO3; EC 1.5.3.17
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-488
  • 氨基酸序列
    MESGGKTNRQ LRKAICVSTD EKMKKKRSPS VIVIGGGMAG ISAARTLQDA SFQVVVLESR DRIGGRVHTD YSFGFPVDLG ASWLHGVCKE NPLAAVIGRL GLPLYRTSGD NSVLYDHDLE SYALFDKAGN QVSQELVTKV GENFEHILEE ICKVRDEQDE DMSIAQAFSI VFKRNPELRL EGLAHNVLQW YLCRMEGWFA ADAETISAKC WDQEELLPGG HGLMVRGYRP VINTLSKGLD IRLSHRITKI SRRYSGVKVT TEKGDTFVAD AAVIALPLGV LKSGMITFEP KLPQWKQEAI NDLGVGIENK IILNFDNVFW PNVEFLGVVA ETSYGCSYFL NLHKATSHPV LVYMPAGQLA RDIEKKSDEA AANFAFSQLQ KILPDASSPI NYLVSRWGSD INSLGSYSYD IVNKPHDLYE RLRVPLDNLF FAGEATSSSY PGSVHGAYST GVLAAEDCRM RVLERYGELE HEMEEEAPAS VPLLISRM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Flavoenzyme involved in polyamine back-conversion. Catalyzes the oxidation of the secondary amino group of polyamines, such as spermine, spermidine and their acetyl derivatives. Substrate preference is spermidine > spermine > N(1)-acetylspermidine > N(1)-acetylspermine. Plays an important role in the regulation of polyamine intracellular concentration (Probable). Involved in the production of hydrogen peroxide during pollen tube growth. Hydrogen peroxide triggers the opening of the hyperpolarization-activated calcium permeable channels in pollen, and thus regulates pollen tube growth.
  • 基因功能参考文献：
    1. Pollen from two mutants lacking expression of AtPAO3 was unable to induce the opening of the Ca(2+) -permeable channels in the presence of Spd, resulting in reduced pollen tube growth and seed number. PMID: 20626657
    2. AtPAO3 catalyzes the sequential conversion/oxidation of spermine to spermidine, and of spermidine to putrescine, thus exhibiting functional homology to the mammalian PAOs. PMID: 18583528
  • 亚细胞定位：
    Peroxisome.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 组织特异性：
    Expressed at similar levels in all tissues tested. Highest expression is seen in siliques.
  • 数据库链接：

    KEGG: ath:AT3G59050

    STRING: 3702.AT3G59050.1

    UniGene: At.27590