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  4. Recombinant Arabidopsis thaliana NADPH-dependent thioredoxin reductase 3 (NTRC)

Recombinant Arabidopsis thaliana NADPH-dependent thioredoxin reductase 3 (NTRC)

  • 中文名称：
    拟南芥NTRC重组蛋白
  • 货号：
    CSB-YP521234DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥NTRC重组蛋白
  • 货号：
    CSB-EP521234DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥NTRC重组蛋白
  • 货号：
    CSB-EP521234DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥NTRC重组蛋白
  • 货号：
    CSB-BP521234DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥NTRC重组蛋白
  • 货号：
    CSB-MP521234DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NTRC
  • Uniprot No.：
  • 别名：
    NTRC; At2g41680; T32G6.20; NADPH-dependent thioredoxin reductase 3; NTR3; EC 1.8.1.9; NADPH-dependent thioredoxin reductase C; ANTR-C; AtNTRC
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    68-529
  • 氨基酸序列
    ATA NSPSSSSSGG EIIENVVIIG SGPAGYTAAI YAARANLKPV VFEGYQMGGV PGGQLMTTTE VENFPGFPDG ITGPDLMEKM RKQAERWGAE LYPEDVESLS VTTAPFTVQT SERKVKCHSI IYATGATARR LRLPREEEFW SRGISACAIC DGASPLFKGQ VLAVVGGGDT ATEEALYLTK YARHVHLLVR RDQLRASKAM QDRVINNPNI TVHYNTETVD VLSNTKGQMS GILLRRLDTG EETELEAKGL FYGIGHSPNS QLLEGQVELD SSGYVLVREG TSNTSVEGVF AAGDVQDHEW RQAVTAAGSG CIAALSAERY LTSNNLLVEF HQPQTEEAKK EFTQRDVQEK FDITLTKHKG QYALRKLYHE SPRVILVLYT SPTCGPCRTL KPILNKVVDE YNHDVHFVEI DIEEDQEIAE AAGIMGTPCV QFFKNKEMLR TISGVKMKKE YREFIEANK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Thioredoxin reductase (TR) that exhibits both TR and thioredoxin (Trx) activities. Contains a C-terminal functional Trx domain. Functions as an electron donor for plastidial 2-Cys peroxiredoxins and participates in a NADPH-dependent hydrogen peroxide scavenging system in chloroplasts in the dark. Required for chlorophyll biosynthesis and biogenesis of the photosynthetic apparatus. Activates aerobic cyclase which converts Mg-protoporhyrin monomethyl ester into protochlorophyllide. Involved in a light-dependent regulation of starch biosynthesis by redox activation of the ADP-glucose pyrophosphorylase (AGPase), a central enzyme of starch synthesis.
  • 基因功能参考文献：
    1. NTRC plays a pivotal role in chloroplast redox regulation, being necessary for the activity of diverse thioredoxins with unrelated functions. PMID: 28500266
    2. NTRC-mediated regulation of the Calvin-Benson cycle and ATP synthesis occurs both directly and through interaction with the ferredoxin-thioredoxin system and is crucial when availability of light is limiting photosynthesis. PMID: 26831830
    3. NTRC is highly sensitive to rapidly changing light intensities.NTRC is not responsible for 'metabolism-related' regulation of the ATP synthase. PMID: 27233821
    4. cooperative control of chloroplast functions via the FTR/Trx and NTRC pathways is essential for plant viability PMID: 27335455
    5. These data uncover a new role for NTRC in the control of photosynthetic yield. PMID: 26476233
    6. An Arabidopsis thaliana double knockout mutant lacking NTRC and Srx shows a phenotype similar to the ntrc mutant, while the srx mutant resembles wild-type plants. NTRC deficiency causes reduced overoxidation of 2-Cys peroxiredoxins. PMID: 25560178
    7. NADPH thioredoxin reductase C is involved in redox regulation of the Mg-chelatase I subunit in Arabidopsis thaliana chloroplasts PMID: 24658415
    8. interaction of chloroplast 2-Cys peroxiredoxin with NADPH-thioredoxin reductase C (NTRC) and thioredoxin x PMID: 25448674
    9. The strongest reduction in ntrc growth occurred under photoperiods with nights longer than 14 h, whereas knockout of the NTRC gene did not alter the circadian-clock-controlled growth. Lack of NTRC modulated chloroplast reactive oxygen species metabolism. PMID: 23881397
    10. heat shock-mediated holdase chaperone function of NTRC is responsible for the increased thermotolerance of Arabidopsis and the activity is significantly supported by NADPH PMID: 23024205
    11. analysis of electron transfer pathways and dynamics of chloroplast NADPH-dependent thioredoxin reductase C (NTRC) PMID: 22833674
    12. NADPH-thioredoxin reductase C (NTRC), previously reported as exclusive to green tissues, is also expressed in nonphotosynthetic tissues of Arabidopsis thaliana. PMID: 22505729
    13. ANTR-C functions as an electron donor for plastidial 2-Cys peroxiredoxins and represents the NADPH-dependent thioredoxin/thioredoxin reductase system in chloroplasts. [ANTR-C] PMID: 16884685
    14. Arabidopsis knock-out mutant showed growth inhibition and hypersensitivity to methyl viologen. PMID: 15292215
    15. NTRC and 2-Cys Prx are involved in the protection of at least the later part of the chlorophyll biosynthetic pathway; NTRC and 2-Cys Prx in vitro stimulate the aerobic cyclase step of this pathway. PMID: 18625226
    16. Chloroplast NADPH-thioredoxin reductase, NTRC, regulates several key processes, including chlorophyll biosynthesis and the shikimate pathway, in chloroplasts. [NTRC] PMID: 19151130

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  • 亚细胞定位：
    Plastid, chloroplast.
  • 蛋白家族：
    Class-II pyridine nucleotide-disulfide oxidoreductase family
  • 数据库链接：

    KEGG: ath:AT2G41680

    STRING: 3702.AT2G41680.1

    UniGene: At.12409