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  4. Recombinant Arabidopsis thaliana Mannose-1-phosphate guanylyltransferase 1 (CYT1)

Recombinant Arabidopsis thaliana Mannose-1-phosphate guanylyltransferase 1 (CYT1)

  • 中文名称：
    Recombinant Arabidopsis thaliana Mannose-1-phosphate guanylyltransferase 1(CYT1),Yeast
  • 货号：
    CSB-YP519497DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Mannose-1-phosphate guanylyltransferase 1(CYT1),Yeast
  • 货号：
    CSB-EP519497DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Mannose-1-phosphate guanylyltransferase 1(CYT1),Yeast
  • 货号：
    CSB-EP519497DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Mannose-1-phosphate guanylyltransferase 1(CYT1),Yeast
  • 货号：
    CSB-BP519497DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Mannose-1-phosphate guanylyltransferase 1(CYT1),Yeast
  • 货号：
    CSB-MP519497DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CYT1
  • Uniprot No.：
  • 别名：
    CYT1; EMB101; GMP1; HSN1; SOZ1; VTC1; At2g39770; T5I7.7Mannose-1-phosphate guanylyltransferase 1; EC 2.7.7.13; GDP-mannose pyrophosphorylase 1; Protein CYTOKINESIS DEFECTIVE 1; Protein EMBRYO DEFECTIVE 101; Protein HYPERSENSITIVE TO AMMONIUM ION 1; Protein SENSITIVE TO OZONE 1; Protein VITAMIN C DEFECTIVE 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-361
  • 氨基酸序列
    MKALILVGGF GTRLRPLTLS FPKPLVDFAN KPMILHQIEA LKAVGVDEVV LAINYQPEVM LNFLKDFETK LEIKITCSQE TEPLGTAGPL ALARDKLLDG SGEPFFVLNS DVISEYPLKE MLEFHKSHGG EASIMVTKVD EPSKYGVVVM EESTGRVEKF VEKPKLYVGN KINAGIYLLN PSVLDKIELR PTSIEKETFP KIAAAQGLYA MVLPGFWMDI GQPRDYITGL RLYLDSLRKK SPAKLTSGPH IVGNVLVDET ATIGEGCLIG PDVAIGPGCI VESGVRLSRC TVMRGVRIKK HACISSSIIG WHSTVGQWAR IENMTILGED VHVSDEIYSN GGVVLPHKEI KSNILKPEIV M
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Plays an essential role in plant growth and development and cell-wall architecture. Provides GDP-mannose, used for cell wall carbohydrate biosynthesis, protein N-glycosylation, as well as for the biosynthesis of the antioxidant ascorbate.
  • 基因功能参考文献：
    1. Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1 have been described. PMID: 27710946
    2. Data reveal that the 27th amino acid of VTC1 confers a key regulation in the interaction with CSN5B and AsA biosynthesis, as well as in Arabidopsis seedling growth. PMID: 27561782
    3. VTC1 is degraded in the dark through the 26S proteasome pathway, and this degradation requires CSN5B. PMID: 23424245
    4. Arabidopsis vtc1 and vtc2 mutants, that have low levels of ascorbate, were more susceptible to Alternaria brassicicola. PMID: 23134520
    5. A mutation in vtc1 causes hypersensitivity to ammonium, resulting in root growth inhibition, altered ammonium metabolism, and hormone homeostasis. PMID: 20007685
    6. Programmed cell death symptoms such as nuclear chromatin condensation, the presence of multivesicular bodies, and extensive degradation and disorganization of the grana stacks were observed in 10-week-old vtc1 leaves. PMID: 16720601

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Transferase hexapeptide repeat family
  • 数据库链接：

    KEGG: ath:AT2G39770

    STRING: 3702.AT2G39770.1

    UniGene: At.10348