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  4. Recombinant Arabidopsis thaliana Gibberellin receptor GID1A (GID1A)

Recombinant Arabidopsis thaliana Gibberellin receptor GID1A (GID1A)

  • 中文名称：
    拟南芥GID1A重组蛋白
  • 货号：
    CSB-YP888945DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥GID1A重组蛋白
  • 货号：
    CSB-EP888945DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥GID1A重组蛋白
  • 货号：
    CSB-EP888945DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥GID1A重组蛋白
  • 货号：
    CSB-BP888945DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥GID1A重组蛋白
  • 货号：
    CSB-MP888945DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GID1A
  • Uniprot No.：
  • 别名：
    GID1A; CXE10; GID1L1; At3g05120; T12H1.8Gibberellin receptor GID1A; EC 3.-.-.-; AtCXE10; Carboxylesterase 10; GID1-like protein 1; Protein GA INSENSITIVE DWARF 1A; AtGID1A
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-345
  • 氨基酸序列
    MAASDEVNLI ESRTVVPLNT WVLISNFKVA YNILRRPDGT FNRHLAEYLD RKVTANANPV DGVFSFDVLI DRRINLLSRV YRPAYADQEQ PPSILDLEKP VDGDIVPVIL FFHGGSFAHS SANSAIYDTL CRRLVGLCKC VVVSVNYRRA PENPYPCAYD DGWIALNWVN SRSWLKSKKD SKVHIFLAGD SSGGNIAHNV ALRAGESGID VLGNILLNPM FGGNERTESE KSLDGKYFVT VRDRDWYWKA FLPEGEDREH PACNPFSPRG KSLEGVSFPK SLVVVAGLDL IRDWQLAYAE GLKKAGQEVK LMHLEKATVG FYLLPNNNHF HNVMDEISAF VNAEC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination, stem elongation and flower development. Partially redundant with GID1B and GID1C.
  • 基因功能参考文献：
    1. stability negatively regulated by ubiquitination and positively regulated by Tyr phosphorylation PMID: 29042542
    2. The active principle of the gibberellin-binding protein is a GID1 homolog. (Review) PMID: 26927225
    3. Partial after-ripening resulted in increased GID1b, but not GID1a or GID1c mRNA levels. PMID: 26136598
    4. activated cytosolic GID1A interacts with DELLA proteins before they enter the nucleus and induces a conformational transition that leads to DELLA's deactivation or degradation in the nucleus PMID: 25104541
    5. The stronger role of GID1b in non-proteolytic gibberellic acid signaling may result from the fact that GID1b has higher affinity for DELLA protein than GID1a and GID1c. PMID: 24521922
    6. GID1A plays a major role during fruit-set, whereas GID1B and GID1C have partially redundant function with GID1A in seed development and pod elongation, respectively. PMID: 24961590
    7. GID1 relieves seed dormancy through the down-regulation of DELLA via direct protein-protein interaction. PMID: 23818171
    8. Computational simulations are revealing a new gibberellin-binding channel of GID1 and a novel hormone perception mechanism involving only one conformational state of GID1. PMID: 23765254
    9. Data show the model of the gene expression feedback regulation of GID1, GA20ox, and GA3ox, and DELLA with gibberellin. PMID: 22523240
    10. Data propose a possible mechanism that might operate for the transcriptional control of GID1 expression by the circadian clock. PMID: 21852756
    11. GID1A, GID1B and GID1C play distinct roles during seed germination. PMID: 21778177
    12. GID1a, GID1b, GID1c are the predominant GA receptors in regulating GA growth response in Arabidopsis. GA binding to GID1a promotes its interaction with DELLA proteins. [GID1a] PMID: 17194763
    13. The double knockout mutant atgid1a atgid1c showed a dwarf phenotype. The stamens of the double knockout mutant atgid1a atgid1b were significantly shorter than those of the wild-type, and this leads to low fertility. PMID: 17521411
    14. DELLA degradation causing GA responses requires GA biosynthesis, three functionally redundant GA receptors GIBBERELLIN INSENSITIVE DWARF1 (GID1a, b, and c), and the SLEEPY1 (SLY1) F-box subunit of an SCF E3 ubiquitin ligase. PMID: 18827182
    15. crystal structure of a ternary complex of Arabidopsis thaliana GID1A, a bioactive gibberellin and the amino-terminal DELLA domain of GAI PMID: 19037309
    16. AtGID1a, AtGID1b and AtGID1c function as GA receptors in Arabidopsis. [AtGID1a] PMID: 16709201

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    'GDXG' lipolytic enzyme family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: ath:AT3G05120

    STRING: 3702.AT3G05120.1

    UniGene: At.18371