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  4. Recombinant Arabidopsis thaliana GDP-L-galactose phosphorylase 1 (VTC2)

Recombinant Arabidopsis thaliana GDP-L-galactose phosphorylase 1 (VTC2)

  • 中文名称：
    拟南芥VTC2重组蛋白
  • 货号：
    CSB-YP823310DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥VTC2重组蛋白
  • 货号：
    CSB-EP823310DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥VTC2重组蛋白
  • 货号：
    CSB-EP823310DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥VTC2重组蛋白
  • 货号：
    CSB-BP823310DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥VTC2重组蛋白
  • 货号：
    CSB-MP823310DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VTC2
  • Uniprot No.：
  • 别名：
    VTC2; At4g26850; F10M23.190GDP-L-galactose phosphorylase 1; EC 2.7.7.69; Protein VITAMIN C DEFECTIVE 2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-442
  • 氨基酸序列
    MLKIKRVPTV VSNYQKDDGA EDPVGCGRNC LGACCLNGAR LPLYACKNLV KSGEKLVISH EAIEPPVAFL ESLVLGEWED RFQRGLFRYD VTACETKVIP GKYGFVAQLN EGRHLKKRPT EFRVDKVLQS FDGSKFNFTK VGQEELLFQF EAGEDAQVQF FPCMPIDPEN SPSVVAINVS PIEYGHVLLI PRVLDCLPQR IDHKSLLLAV HMAAEAANPY FRLGYNSLGA FATINHLHFQ AYYLAMPFPL EKAPTKKITT TVSGVKISEL LSYPVRSLLF EGGSSMQELS DTVSDCCVCL QNNNIPFNIL ISDCGRQIFL MPQCYAEKQA LGEVSPEVLE TQVNPAVWEI SGHMVLKRKE DYEGASEDNA WRLLAEASLS EERFKEVTAL AFEAIGCSNQ EEDLEGTIVH QQNSSGNVNQ KSNRTHGGPI TNGTAAECLV LQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants. Acts as a phosphorylase rather than as a transferase. Uses preferentially GDP-L-galactose and GDP-D-glucose as substrates. Lower activity with GDP-L-fucose, very low activity with GDP-D-mannose, and no activity with UDP-D-glucose, UDP-D-galactose or ADP-D-glucose. Highly specific for inorganic phosphate as the guanylyl acceptor.
  • 基因功能参考文献：
    1. GGP/VTC2 acts as a rate-limiting step regulating AsA biosynthesis in response to light and dark conditions PMID: 25036484
    2. The VTC5 promoter is more active in young cotyledons; the VTC2 and VTC5 promoters for two GDP-L-galactose phosphorylase isozymes drive a day-to-night variation in expression. PMID: 21897033
    3. A high proportion of the primary roots of the vtc2 plants grown on soil had lost the wild-type responses to gravity. PMID: 16720601
    4. VTC2 from A. thaliana is a specific GDP-L-galactose/GDP-D-glucose phosphorylase PMID: 17462988
    5. At4g26850 codes for GDP-L-galactose-hexose-1-phosphate guanyltransferase which catalyzing the transfer of GMP from GDP-L-galactose to a hexose-1-P, most likely D-mannose-1-phosphate in vivo. [galactose guanyltransferase] PMID: 17485667
    6. Data show that a GDP-L-galactose phosphorylase, encoded by the Arabidopsis thaliana VTC2 gene, catalyses this step in the ascorbate biosynthetic pathway. PMID: 17877701
    7. Arabidopsis VTC2 and VTC5 proteins are enzymes that guanylylate a conserved active site His residue with GDP-L-galactose, forming L-galactose 1-phosphate for vitamin C synthesis, and regenerate the enzyme with phosphate to form GDP. PMID: 18463094
    8. The VTC2 gene was expressed in all developmental stages, and the mRNA was expressed at a higher level in green tissues than in the root. PMID: 18516687

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    GDPGP1 family
  • 组织特异性：
    Expressed in leaves, stems, roots, flowers and siliques. Highest expression in green tissues.
  • 数据库链接：

    KEGG: ath:AT4G26850

    STRING: 3702.AT4G26850.1

    UniGene: At.23783