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  4. Recombinant Arabidopsis thaliana G-protein coupled receptor 1 (GCR1), partial

Recombinant Arabidopsis thaliana G-protein coupled receptor 1 (GCR1), partial

  • 中文名称：
    拟南芥GCR1重组蛋白
  • 货号：
    CSB-YP009728DOA1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥GCR1重组蛋白
  • 货号：
    CSB-EP009728DOA1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥GCR1重组蛋白
  • 货号：
    CSB-EP009728DOA1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥GCR1重组蛋白
  • 货号：
    CSB-BP009728DOA1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥GCR1重组蛋白
  • 货号：
    CSB-MP009728DOA1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GCR1
  • Uniprot No.：
  • 别名：
    GCR1; At1g48270; F11A17.17; G-protein coupled receptor 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Together with GPA1, may regulate the cell cycle via a signaling cascade that uses phosphatidylinositol-specific phospholipase C (PI-PLC) as an effector and inositol 1,4,5-trisphosphate(IP(3)) as a second messenger. Promotes PI-PLC activity and IP(3) accumulation. Involved in the blue light (BL) signaling. Together with GPA1 and ADT3, required for BL-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe), in etiolated seedlings. Probably involved in cytokinin signal transduction. Plays a positive role in gibberellin- (GA) and brassinosteroid- (BR) regulated seed germination, probably independently of a heterotrimeric G-protein. Mediates seed dormancy abolition, and promotes seed germination and flowering.
  • 基因功能参考文献：
    1. provide compelling genetic evidence to revisit the role of GCR1 in G-protein signalling PMID: 26346778
    2. GCR1 may act as a negative regulator of G protein alpha subunit GPA1-mediated abscisic acid responses in guard cells [GCR1] PMID: 15155892
    3. GCR1 plays a positive role in gibberellin and brassinosteroid regulated seed germination. Unlike a typical 7TM receptor, it acts independently of the heterotrimeric G-protein. PMID: 15181210
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Note=Localized to the outer edge of the leaf epidermal cells in a punctuate pattern.
  • 蛋白家族：
    G-protein coupled receptor 2 family
  • 组织特异性：
    Mostly present in the meristematic regions. Expressed at low levels in seedlings, vascular tissues of cotyledons, hypocotyl, and roots, stems, leaves, flowering buds and siliques. In dark-grown seedlings, localized in the cotyledons and the hook.
  • 数据库链接：

    KEGG: ath:AT1G48270

    STRING: 3702.AT1G48270.1

    UniGene: At.21469