Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Arabidopsis thaliana Eukaryotic initiation factor 4A-1 (TIF4A-1)

Recombinant Arabidopsis thaliana Eukaryotic initiation factor 4A-1 (TIF4A-1)

  • 货号：
    CSB-YP334836DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP334836DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP334836DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP334836DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP334836DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TIF4A-1
  • Uniprot No.：
  • 别名：
    EIF4A1; RH4; TIF4A-1; At3g13920; MDC16.4Eukaryotic initiation factor 4A-1; eIF-4A-1; EC 3.6.4.13; ATP-dependent RNA helicase eIF4A-1; DEAD-box ATP-dependent RNA helicase 4; AtRH04
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-412
  • 氨基酸序列
    MAGSAPEGTQ FDARQFDQKL NEVLEGQDEF FTSYDDVHES FDAMGLQENL LRGIYAYGFE KPSAIQQRGI VPFCKGLDVI QQAQSGTGKT ATFCSGVLQQ LDFSLIQCQA LVLAPTRELA QQIEKVMRAL GDYLGVKVHA CVGGTSVRED QRILQAGVHV VVGTPGRVFD MLKRQSLRAD NIKMFVLDEA DEMLSRGFKD QIYDIFQLLP PKIQVGVFSA TMPPEALEIT RKFMSKPVRI LVKRDELTLE GIKQFYVNVE KEEWKLETLC DLYETLAITQ SVIFVNTRRK VDWLTDKMRS RDHTVSATHG DMDQNTRDII MREFRSGSSR VLITTDLLAR GIDVQQVSLV INFDLPTQPE NYLHRIGRSG RFGRKGVAIN FVTRDDERML FDIQKFYNVV VEELPSNVAD LL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • 基因功能参考文献：
    1. Cyclin-dependent kinase A (CDKA) phosphorylates eukaryotic initiation factor 4A (eIF4A)eIF4A1 and eIF4A2 on a conserved threonine residue (threonine-164) within the RNA-binding motif. PMID: 27388680
    2. EIF4A function is essential for plant growth and development in Arabidopsis. PMID: 26493293
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    DEAD box helicase family, eIF4A subfamily
  • 组织特异性：
    Highly expressed in the whole plant.
  • 数据库链接：

    KEGG: ath:AT3G13920

    UniGene: At.23558