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  4. Recombinant Arabidopsis thaliana Developmental protein SEPALLATA 3 (SEP3)

Recombinant Arabidopsis thaliana Developmental protein SEPALLATA 3 (SEP3)

  • 中文名称：
    Recombinant Arabidopsis thaliana Developmental protein SEPALLATA 3(SEP3),Yeast
  • 货号：
    CSB-YP515777DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Developmental protein SEPALLATA 3(SEP3),Yeast
  • 货号：
    CSB-EP515777DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Developmental protein SEPALLATA 3(SEP3),Yeast
  • 货号：
    CSB-EP515777DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Developmental protein SEPALLATA 3(SEP3),Yeast
  • 货号：
    CSB-BP515777DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Developmental protein SEPALLATA 3(SEP3),Yeast
  • 货号：
    CSB-MP515777DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SEP3
  • Uniprot No.：
  • 别名：
    SEP3; AGL9; At1g24260; F3I6.19; Developmental protein SEPALLATA 3; Agamous-like MADS-box protein AGL9
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-251
  • 氨基酸序列
    MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN RGKLYEFCSS SSMLRTLERY QKCNYGAPEP NVPSREALAV ELSSQQEYLK LKERYDALQR TQRNLLGEDL GPLSTKELES LERQLDSSLK QIRALRTQFM LDQLNDLQSK ERMLTETNKT LRLRLADGYQ MPLQLNPNQE EVDHYGRHHH QQQQHSQAFF QPLECEPILQ IGYQGQQDGM GAGPSVNNYM LGWLPYDTNS I
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development. Binds specifically to the CArG box DNA sequence 5'-CC (A/T)6 GG-3'.
  • 基因功能参考文献：
    1. Cloning, prokaryotic expression and purification of CpfS1 (Sep3) protein from Arabidopsis thaliana has ben described. PMID: 26718440
    2. s analyzed the oligomeric status of SEP3 domains in the absence of the DNA-binding MADS-box domain PMID: 26505403
    3. The 2.5-A crystal structure of a small portion of the intervening and the complete keratin-like domain of SEP3, is reported. PMID: 25228343
    4. SEP3 is a member of the MADS transcription factor family and plays a role in the development of the floral organs through the formation of multiprotein complexes with other MADS-family transcription factors. PMID: 23989147
    5. Here, we show that SEP3 as a downstream gene of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 (SPL3) and FT modulates the flowering time in response to different ambient temperatures. PMID: 22899051
    6. Approximately 90% of the binding sites of two well-characterized MADS domain transcription factors, APETALA1 and SEPALLATA3, were covered by the DNase I hypersensitive (DH) sites. PMID: 22773751
    7. higher-order complex formation is a general and essential molecular mechanism for plant MADS box protein functioning and SEP3 'glue' protein plays a pivotal role in mediating multimerization PMID: 19243611
    8. In order to uncover new roles of SEP3 we studied in detail the phenotypes produced by 35S::SEP3 in different wild type and mutant backgrounds. [SEPALLATA3] [SEP3] PMID: 16098111
    9. Evidence that in vitro homotetramers of the class E floral homeotic protein SEPALLATA3 from Arabidopsis thaliana bind cooperatively to two sequence elements termed 'CArG boxes' in a phase-dependent manner involving DNA looping. PMID: 19033361
    10. SEPALLATA3 integrates and modulates different growth-related and hormonal pathways in a combinatorial fashion with other MADS-box proteins and possibly with non-MADS transcription factors. PMID: 19385720

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: ath:AT1G24260

    STRING: 3702.AT1G24260.2

    UniGene: At.24086