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  4. Recombinant Arabidopsis thaliana Cytochrome P450 85A2 (CYP85A2), partial

Recombinant Arabidopsis thaliana Cytochrome P450 85A2 (CYP85A2), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 85A2(CYP85A2),partial,Yeast
  • 货号：
    CSB-YP846372DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 85A2(CYP85A2),partial,Yeast
  • 货号：
    CSB-EP846372DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 85A2(CYP85A2),partial,Yeast
  • 货号：
    CSB-EP846372DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 85A2(CYP85A2),partial,Yeast
  • 货号：
    CSB-BP846372DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Cytochrome P450 85A2(CYP85A2),partial,Yeast
  • 货号：
    CSB-MP846372DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CYP85A2
  • Uniprot No.：
  • 别名：
    CYP85A2; BR6OX2; At3g30180; T20F20.9; T20F20_6Cytochrome P450 85A2; EC 1.14.-.-; Brassinosteroid-6-oxidase 2; BR6ox 2; C6-oxidase 2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the C6-oxidation step in brassinosteroids biosynthesis. Converts 6-deoxocastasterone to castasterone, and castasterone to brassinolide. May also convert 6-deoxoteasterone to teasterone, 3-dehydro-6-deoxoteasterone to 3-dehydroteasterone, and 6-deoxotyphasterol to typhasterol.
  • 基因功能参考文献：
    1. CYP85A2, the cytochrome P450 enzyme that performs the last step in brassinosteroid biosynthesis (conversion of castasterone to brassinolide)(4), must be farnesylated to function in Arabidopsis. Loss of either CYP85A2 or CYP85A2 farnesylation results in reduced brassinolide accumulation and increased plant responsiveness to the hormone abscisic acid (ABA) and overall drought tolerance, explaining previous observations. PMID: 27455172
    2. The seu cyp85A2 double mutants displayed enhanced disruptions of gynoecial and ovule development. PMID: 20836864
    3. investigated the temporal and spatial expression patterns of CYP85A1 and CYP85A2; both promoters of CYP85A1 and CYP85A2 showed maximum activity during the first week following germination, particularly in the vascular tissues PMID: 15821873
    4. CYP85A2 is a bifunctional enzyme that possesses BR C-6 oxidase (C-6 oxidation of brassinosteroids) and brassinolide (BL) synthase activity. It is thus a cytochrome P450 that mediates Baeyer-Villiger oxidation in plants. [CYP85A2] PMID: 16024588
    5. CYP85A2 catalyzes the lactonization reactions of not only castasterone but also teasterone and typhasterol PMID: 18685225

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  • 亚细胞定位：
    Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Expressed in stems, leaves, siliques, shoots, and roots, with a higher expression in apical shoots.
  • 数据库链接：

    KEGG: ath:AT3G30180

    STRING: 3702.AT3G30180.1

    UniGene: At.26493