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  4. Recombinant Arabidopsis thaliana B3 domain-containing transcription factor LEC2 (LEC2)

Recombinant Arabidopsis thaliana B3 domain-containing transcription factor LEC2 (LEC2)

  • 中文名称：
    拟南芥LEC2重组蛋白
  • 货号：
    CSB-YP631971DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥LEC2重组蛋白
  • 货号：
    CSB-EP631971DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥LEC2重组蛋白
  • 货号：
    CSB-EP631971DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥LEC2重组蛋白
  • 货号：
    CSB-BP631971DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥LEC2重组蛋白
  • 货号：
    CSB-MP631971DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LEC2
  • Uniprot No.：
  • 别名：
    LEC2; At1g28300; F3H9.5B3 domain-containing transcription factor LEC2; Protein LEAFY COTYLEDON 2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-363
  • 氨基酸序列
    MDNFLPFPSS NANSVQELSM DPNNNRSHFT TVPTYDHHQA QPHHFLPPFS YPVEQMAAVM NPQPVYLSEC YPQIPVTQTG SEFGSLVGNP CLWQERGGFL DPRMTKMARI NRKNAMMRSR NNSSPNSSPS ELVDSKRQLM MLNLKNNVQI SDKKDSYQQS TFDNKKLRVL CEKELKNSDV GSLGRIVLPK RDAEANLPKL SDKEGIVVQM RDVFSMQSWS FKYKFWSNNK SRMYVLENTG EFVKQNGAEI GDFLTIYEDE SKNLYFAMNG NSGKQNEGRE NESRERNHYE EAMLDYIPRD EEEASIAMLI GNLNDHYPIP NDLMDLTTDL QHHQATSSSM PPEDHAYVGS SDDQVSFNDF EWW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription regulator that plays a central role in embryo development. Required for the maintenance of suspensor morphology, specification of cotyledon identity, progression through the maturation phase and suppression of premature germination. Ectopic expression is sufficient to promote somatic embryogenesis.
  • 基因功能参考文献：
    1. senescence-inducible and triggers the key metabolic steps that increase triacylglycerol accumulation in leaves PMID: 25790072
    2. LEC2 overexpression modifies the morphology and anatomy of leaves by promoting differentiation toward an embryonic pathway. PMID: 23780897
    3. LEC2 alters: (1) the delivery of photosynthates from the seed coat to the embryo, (2) carbon partitioning towards different storage compounds, (3) the rate of starch synthesis, and (4) degradation in developing seeds and germination capacity of dry seeds. PMID: 21665323
    4. Transcriptional regulation of Arabidopsis LEAFY COTYLEDON2 involves a cis-element that regulates trimethylation of histone H3 at lysine-27. PMID: 22080598
    5. Role and expression pattern of LEC2 in seed development PMID: 22228409
    6. revealed differential expression of LEC2 transcripts within a 30 days time course of somatic embryo development. PMID: 19763577
    7. Loss of embryogenic potential in the lec2 mutant in vitro is not related to the distribution of exogenously applied auxin. PMID: 16034595
    8. Data show that the induction of LEAFY COTYLEDON 2 leads to the accumulation of storage oil and seed specific mRNAs in leaves, and increases the expression of the seed regulators namely, LEC1, FUS3, and ABI3. PMID: 16107256
    9. demonstrate that LEC2 directly controls a transcriptional program involved in the maturation phase of seed development PMID: 16492731
    10. WRINKLED 1 is a target of LEAFY COTYLEDON2 and is necessary for the regulatory action on fatty acid metabolism. [Leafy Cotyledon2] [LEC2] PMID: 17419836
    11. LEC2 causes rapid changes in auxin responses and induces cellular differentiation characteristic of the maturation phase. PMID: 18287041

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: ath:AT1G28300

    STRING: 3702.AT1G28300.1

    UniGene: At.26290