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  4. Recombinant Acinetobacter baumannii Beta-lactamase (NDM-1), partial

Recombinant Acinetobacter baumannii Beta-lactamase (NDM-1), partial

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  • 中文名称：
    Recombinant Acinetobacter baumannii Beta-lactamase(NDM-1),partial
  • 货号：
    CSB-EP761ABS
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP076174Ba could indicate that this peptide derived from E.coli-expressed Acinetobacter baumannii ndm-1.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP076174Ba could indicate that this peptide derived from E.coli-expressed Acinetobacter baumannii ndm-1.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • Uniprot No.：
  • 种属：
    Acinetobacter baumannii
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    22.0kDa
  • 表达区域：
    164-222aa
  • 氨基酸序列
    AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The DNA coding sequence translated into the Acinetobacter baumannii NDM-1 protein sequence (164-222aa) was fused with the N-terminal 6xHis-SUMO tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant Acinetobacter baumannii NDM-1 protein with N-terminal 6xHis-SUMO tag. The SDS-PAGE analysis determined its purity higher than 90%. After electrophoresis, a 21 kDa protein band was observed on the gel.

    NDM-1 is a gene providing instructions for making a protein Beta-lactamase in Acinetobacter baumannii. This gene is a novel metallo-beta-lactamase (MBL) gene carried by some Enterobacteriaceae that induces resistance to most of the antibiotics. This gene was first describled in a Swedish patient hospitalized in India with an infection due to Klebsiella pneumoniae. NDM-1 makes bacteria resistant to a broad range of beta-lactam antibiotics, including the antibiotics of the carbapenem family (a mainstay for the treatment of antibiotic-resistant bacterial infections).

  • Datasheet & COA：
    Please contact us to get it.

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