Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Acidaminococcus fermentans (R)-2-hydroxyglutaryl-CoA dehydratase subunit alpha

Recombinant Acidaminococcus fermentans (R)-2-hydroxyglutaryl-CoA dehydratase subunit alpha

  • 货号：
    CSB-YP320884AWA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP320884AWA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP320884AWA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP320884AWA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP320884AWA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    hgdA
  • Uniprot No.：
  • 别名：
    hgdA; Acfer_1815(R)-2-hydroxyglutaryl-CoA dehydratase; subunit alpha; EC 4.2.1.167,; R)-2-hydroxyglutaryl-CoA dehydratase; component D
  • 种属：
    Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-477
  • 氨基酸序列
    PKTVSPGVQ ALRDVVEKVY RELREAKERG EKVGWSSSKF PCELAESFGL HVGYPENQAA GIAANRDGEV MCQAAEDIGY DNDICGYARI SLAYAAGFRG ANKMDKDGNY VINPHSGKQM KDANGKKVFD ADGKPVIDPK TLKPFATTDN IYEIAALPEG EEKTRRQNAL HKYRQMTMPM PDFVLCCNNI CNCMTKWYED IARRHNIPLI MIDVPYNEFD HVNEANVKYI RSQLDTAIRQ MEEITGKKFD EDKFEQCCQN ANRTAKAWLK VCDYLQYKPA PFNGFDLFNH MADVVTARGR VEAAEAFELL AKELEQHVKE GTTTAPFKEQ HRIMFEGIPC WPKLPNLFKP LKANGLNITG VVYAPAFGFV YNNLDELVKA YCKAPNSVSI EQGVAWREGL IRDNKVDGVL VHYNRSCKPW SGYMPEMQRR FTKDMGIPTA GFDGDQADPR NFNAAQYETR VQGLVEAMEA NDEKKGK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the fermentation of L-glutamate via the hydroxyglutarate pathway. Catalyzes the reversible syn-elimination of water from (R)-2-hydroxyglutaryl-CoA to yield (E)-glutaconyl-CoA. The dehydration mechanism involves a transient one electron reduction of the thioester from (R)-2-hydroxyglutaryl-CoA, generating a ketyl radical. Prior to (E)-glutaconyl-CoA formation, the ketyl radical is subsequently reoxidized by electron transfer back to the HgdA-HgdB complex (CompD) to avoid change in oxidation state of the substrate. The appropriate redox state of dehydratase HgdA-HgdB complex (CompD) is maintained by HgdC (CompA) via hydrolysis of ATP and ATP-dependent electron transfer. Since the electron is recycled, the dehydratase is able to perform several turnovers with only catalytic amounts of ATP and substoichiometric amounts of HgdC (CompA).
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    FldB/FldC dehydratase alpha/beta subunit family
  • 数据库链接：

    KEGG: afn:Acfer_1815

    STRING: 591001.Acfer_1815