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SRPK2 Antibody

  • 中文名称：
    SRPK2兔多克隆抗体
  • 货号：
    CSB-PA022683LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • IHC image of CSB-PA022683LA01HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate
      All lanes: SRPK2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 78, 80 kDa
      Observed band size: 78 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) SRPK2 Polyclonal antibody
  • Uniprot No.：
    P78362
  • 基因名：
    SRPK2
  • 别名：
    Human serine kinase SRPK2 mRNA, complete cds antibody; Serine kinase SRPK2 antibody; Serine/arginine rich protein specific kinase 2 antibody; Serine/arginine-rich protein-specific kinase 2 antibody; Serine/threonine protein kinase SRPK2 antibody; SFRS protein kinase 2 antibody; SFRSK2 antibody; SR protein specific kinase 2 antibody; SR-protein-specific kinase 2 antibody; SRPK2 antibody; SRPK2_HUMAN antibody; SRSF protein kinase 2 C-terminal antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human SRSF protein kinase 2 protein (471-688AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，SRPK2 Antibody (CSB-PA022683LA01HU)，的标记方式是Non-conjugated。对于SRPK2 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA022683LB01HU SRPK2 Antibody, HRP conjugated ELISA
    FITC CSB-PA022683LC01HU SRPK2 Antibody, FITC conjugated
    Biotin CSB-PA022683LD01HU SRPK2 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    Antigen Affinity Purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Probably by phosphorylating DDX23, leads to the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles.
  • 基因功能参考文献：
    1. SRPK2 may play an important role in the progression and metastasis of prostate cancer. PMID: 29587239
    2. pausing of RNA polymerase II (RNA Pol II) initiates a signaling cascade whereby the serine/arginine protein kinase 2 (SRPK2) phosphorylates the DDX23 helicase, culminating in the suppression of R-loops. PMID: 28076779
    3. ten candidate variants were prioritised. Of these, SRPK2 (c.2044C>T[p.Arg682Trp]) and NOTCH1 (c.3835C>T[p.Arg1279Cys]), co-segregated with disease in the family; however, previous functional analyses on SRPK2 make this an unlikely candidate. Functional analyses in the variant (c.3835C>T[p.Arg1279Cys]) of the known CHD gene NOTCH1 demonstrated a non-significant decrease in signalling activity. PMID: 27989580
    4. Delta-secretase phosphorylation by SRPK2 plays a critical role in aggravating Alzheimer disease pathogenesis. PMID: 28826672
    5. In this study, it was found that the expression of SRPK2 was up-regulated in the clinical colon cancer samples. Overexpression of SRPK2 promoted the growth and migration of colon cancer cells, while knocking down the expression of SRPK2 inhibited the growth, migration and tumorigenecity of colon cancer cells. PMID: 27041240
    6. Our results indicate that phosphorylation of SRPK2 plays a crucial role in the regulation of splicing process in head and neck squamous cell carcinoma (HNSCC), and that splicing kinases can be developed as a new class of therapeutic target in HNSCC. PMID: 26853621
    7. A conserved electronegative docking groove in SRPK2 is responsible for substrate binding. PMID: 24444330
    8. the BLRF2 RS motif is phosphorylated by SRPK2 and is important for viral replication. PMID: 23326445
    9. Short-term exercise resulted in a significant increase of mRNA expression of genes encoding proteins involved in the formation of precatalytic splisosome: SRPK2. PMID: 19902070
    10. SRPK2 knock down results in hypophosphorylation of the arginine-serine (RS) domain-containing human PRP28 protein (PRP28, also known as DDX23), and destabilizes PRP28 association with the tri-snRNP. PMID: 18425142
    11. overexpression of SRPK2 is associated with phosphorylating acinus and regulating its stimulatory effects on cyclin A1 expression, contributing to leukemia cell proliferation PMID: 18559500
    12. Interaction of Akt-phosphorylated SRPK2 with 14-3-3 mediates cell cycle and cell death in neurons. PMID: 19592491

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  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleoplasm. Nucleus speckle. Chromosome.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family
  • 组织特异性：
    Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.
  • 数据库链接：

    HGNC: 11306

    OMIM: 602980

    KEGG: hsa:6733

    STRING: 9606.ENSP00000377262

    UniGene: Hs.285197