Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Roundabout homolog 4(ROBO4) ELISA kit

Mouse Roundabout homolog 4(ROBO4) ELISA kit

  • 中文名称：
    小鼠环形交叉轴突导向受体同源物4(ROBO4)酶联免疫试剂盒
  • 货号：
    CSB-EL020057MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse ROBO4 ELISA Kit was designed for the quantitative measurement of Mouse ROBO4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Robo4 ELISA Kit; Roundabout homolog 4 ELISA Kit
  • 缩写：
    ROBO4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ROBO4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 81-89
    1:2 Average % 98
    Range % 94-103
    1:4 Average % 86
    Range % 83-89
    1:8 Average % 98
    Range % 95-102
  • 回收率：
    The recovery of mouse ROBO4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 101-109
    EDTA plasma (n=4) 89 85-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.524 2.631 2.578 2.404
    5 1.957 1.904 1.931 1.757
    2.5 1.240 1.304 1.272 1.098
    1.25 0.753 0.768 0.761 0.587
    0.625 0.496 0.511 0.504 0.330
    0.312 0.302 0.320 0.311 0.137
    0.156 0.220 0.234 0.227 0.053
    0 0.176 0.172 0.174 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function.
  • 基因功能参考文献：
    1. Robo4 inhibits angiogenesis and vessel permeability independently of its cytoplasmic domain. Robo4 deletion enhances permeability and revascularization in oxygen-induced retinopathy (OIR) and accelerates cutaneous wound healing. PMID: 27882935
    2. endothelial Robo4 signaling is important for suppressing breast cancer growth and metastasis, and it can be targeted (enhanced) by administrating a small molecular drug. PMID: 26778715
    3. Robo4 vaccines induce antibodies that retard tumor growth. PMID: 25348086
    4. Robo4 and Src family kinases are involved in the mechanisms of embryonic vascular defects caused by the Hrt/Hey deficiency. PMID: 25264302
    5. These results clearly indicate that Robo4 plays a role in hematopoietic stem cells trafficking such as bone marrow homing and peripheral mobilization. PMID: 23226403
    6. our results showed an important role of Robo4 in the regulation of cortical radial migration through Slit-dependent and -independent mechanisms PMID: 22123939
    7. Data found that expression of Slit2 and SDF1 were dynamically regulated in mesenchymal stem cells and OBC-like populations following radiation, while Robo4 expression was restricted to endothelial cells. PMID: 22313734
    8. augmenting the host Robo4/Slit2 system could provide a useful therapeutic approach to control pathological angiogenesis associated with HSV induced stromal keratitis PMID: 21572022
    9. Robo4 specifically binds to UNC5B, a vascular Netrin receptor, revealing unexpected interactions between two endothelial guidance receptors. PMID: 21238923
    10. Activating with the soluble ligand Slit an endothelium-specific, Robo4-dependent signaling pathway that strengthens the vascular barrier, diminishing deleterious aspects of the host's response to the pathogen-induced cytokine storm. PMID: 20375003
    11. enhanced growth of blood vessels in Robo4(-/-) endothelium is due to activation of vascular endothelial growth factor (VEGF)-R2 signaling through the Src and FAK kinases. PMID: 20498081
    12. Robo4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. PMID: 12941633
    13. Robo4 is expressed in hematopoietic stem cells (HSC), and Robo4/Slit2 signaling may play a role in HSC homeostasis in the bone marrow niche. PMID: 18927479
    14. Robo4 mRNA level decreases during vascular decline in pulmonary fibrosis. PMID: 17496152

    显示更多

    收起更多

  • 蛋白家族：
    Immunoglobulin superfamily, ROBO family
  • 组织特异性：
    Expressed specifically in embryo and adult vascular endothelium.
  • 数据库链接：