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  4. Mouse Hepatocyte growth factor activator(HGFAC) ELISA kit

Mouse Hepatocyte growth factor activator(HGFAC) ELISA kit

  • 中文名称：
    小鼠肝细胞生长因子激活物(HGFAC)酶联免疫试剂盒
  • 货号：
    CSB-EL010328MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse HGFAC ELISA Kit was designed for the quantitative measurement of Mouse HGFAC protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

  • 别名：
    Hgfac ELISA Kit; Hepatocyte growth factor activator ELISA Kit; HGF activator ELISA Kit; HGFA ELISA Kit; EC 3.4.21.-) [Cleaved into: Hepatocyte growth factor activator short chain; Hepatocyte growth factor activator long chain] ELISA Kit
  • 缩写：
    HGFAC
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse HGFAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 104
    Range % 98-109
    1:2 Average % 97
    Range % 90-101
    1:4 Average % 92
    Range % 86-95
    1:8 Average % 97
    Range % 93-102
  • 回收率：
    The recovery of mouse HGFAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 87-98
    EDTA plasma (n=4) 98 92-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.580 2.469 2.525 2.355
    1000 1.826 1.902 1.864 1.694
    500 1.203 1.183 1.193 1.023
    250 0.682 0.664 0.673 0.503
    125 0.399 0.385 0.392 0.222
    62.5 0.277 0.284 0.281 0.111
    31.25 0.203 0.209 0.206 0.036
    0 0.169 0.171 0.170 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.
  • 基因功能参考文献：
    1. HGFA is required for repair of injured intestinal mucosa but is not essential for normal development during embryogenesis or after birth. PMID: 15521012
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：