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Fish hepatocyte growth factor(HGF)ELISA Kit

  • 中文名称:
    鱼肝细胞生长因子(HGF)酶联免疫试剂盒
  • 货号:
    CSB-E16527Fh
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 缩写:
  • Uniprot No.:
  • 种属:
    Fish
  • 样本类型:
    serum, plasma
  • 检测范围:
    200 pg/mL-8000 pg/mL
  • 灵敏度:
    167 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 95
    Range % 89-105
    1:2 Average % 89
    Range % 85-100
    1:4 Average % 89
    Range % 82-100
    1:8 Average % 93
    Range % 87-99
  • 回收率:
    The recovery of fish HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 93 88-98
    EDTA plasma (n=4) 95 89-102
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average  
    8000 0.176 0.182 0.179  
    4000 0.381 0.379 0.380  
    2000 0.558 0.565 0.562  
    685 0.886 0.902 0.894  
    230 1.502 1.488 1.495  
    0 2.415 2.436 2.426  
  • 数据处理:
  • 货期:
    3-5 working days

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