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Fish (Salmon) leptin (LEP) ELISA kit

  • 中文名称:
    鱼(鲑鱼)瘦素(LEP)酶联免疫试剂盒
  • 货号:
    CSB-EL012870FI
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:

    This Fish LEP ELISA Kit was designed for the quantitative measurement of Fish LEP protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 31.25 pg/mL.

  • 缩写:
  • Uniprot No.:
  • 种属:
    Fish(Salmon)
  • 样本类型:
    serum, plasma
  • 检测范围:
    62.5 pg/mL-1000 pg/mL
  • 灵敏度:
    31.25 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 91  
    Range % 86-98  
    1:40 Average % 97  
    Range % 91-102  
    1:80 Average % 84  
    Range % 80-87  
    1:160 Average % 90  
    Range % 83-96  
  • 回收率:
    The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-96  
    EDTA plasma (n=4) 97 93-102  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.082 0.086 0.084    
    500 0.190 0.200 0.195    
    250 0.483 0.444 0.464    
    125 0.945 0.959 0.952    
    62.5 1.672 1.555 1.614    
    0 2.591 2.670 2.631    
  • 数据处理:
  • 货期:
    3-5 working days

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问答及客户评论

 常见问题解答
Q:

https://www.cusabio.com/ELISA-Kit/Fish-Salmon-leptin-LEP-ELISA-kit-87315.html
Have you ever tested tissue sample?

A:
Thanks for your inquiry!We only tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test tissue samplet,we suggest do pre-testing first.Here is the processing way on tissue samples.Pls check for your reference:Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After ...展开 >>
Q:

Is this kit also compatible to test Leptin levels in Zebrafish?

A:
Thanks for your inquiry!We suggest you do a pretest if you want to test zebrafish.