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中文名称:鱼(鲑鱼)瘦素(LEP)酶联免疫试剂盒
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货号:CSB-EL012870FI
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:
This Fish LEP ELISA Kit was designed for the quantitative measurement of Fish LEP protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-1000 pg/mL and the sensitivity is 31.25 pg/mL.
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缩写:
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Uniprot No.:
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种属:Fish(Salmon)
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样本类型:serum, plasma
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检测范围:62.5 pg/mL-1000 pg/mL
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灵敏度:31.25 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Metabolism
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 91 Range % 86-98 1:40 Average % 97 Range % 91-102 1:80 Average % 84 Range % 80-87 1:160 Average % 90 Range % 83-96 -
回收率:
The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-96 EDTA plasma (n=4) 97 93-102 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 1000 0.082 0.086 0.084 500 0.190 0.200 0.195 250 0.483 0.444 0.464 125 0.945 0.959 0.952 62.5 1.672 1.555 1.614 0 2.591 2.670 2.631 -
数据处理:
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货期:3-5 working days
引用文献
- Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus) RE Ibrahim,/,2024
- Sequence identification and expression characterization of leptin in the spotted scat, Scatophagus argus WX Li,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
- The feedback regulation of carbohydrates intake on food intake and appetite in grass carp (Ctenopharyngodon idella) XC Yuan,Fish Physiol Biochem,2021
- Lipid Deposition and Mobilisation in Atlantic Salmon Adipocytes M Bou,international journal of molecular sciences,2020
- Comparative analysis of glucose metabolism responses of large yellow croaker Larimichthys crocea fed diet with fish oil and palm oil Wang Q,Fish Physiology and Biochemistry,2019
- High level of dietary soybean oil affects the glucose and lipid metabolism in large yellow croaker Larimichthys crocea through the insulin-mediated PI3K/AKT signaling pathway Gu Z, et al,Comparative Biochemistry And Physiology B-Biochemistry & Molecular Biology,2019
- Stress, cortisol, and feed intake in rainbow trout (Oncorhynchus mykiss) - Can you stomach the stress? Hoyland, Maren.et al,/,2018
- Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
- Physiological responses of Atlantic salmon (Salmo salar L.) fed very low (3%) fishmeal diets supplemented with feeding-modulating crystalline amino acid Katerina Kousoulaki.et al,Aquaculture,2017
- Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat Rong Yao, et al,PLoS One.,2015
问答及客户评论
■ 常见问题解答
Q:
https://www.cusabio.com/ELISA-Kit/Fish-Salmon-leptin-LEP-ELISA-kit-87315.html
Have you ever tested tissue sample?
A:
Thanks for your inquiry!We only tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test tissue samplet,we suggest do pre-testing first.Here is the processing way on tissue samples.Pls check for your reference:Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After ...展开 >>
Q:
Is this kit also compatible to test Leptin levels in Zebrafish?
A:
Thanks for your inquiry!We suggest you do a pretest if you want to test zebrafish.