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  4. Recombinant Human Ceramide synthase 2 (CERS2)

Recombinant Human Ceramide synthase 2 (CERS2)

  • 货号：
    CSB-CF839326HU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    CERS2
  • Uniprot No.：
  • 别名：
    CERS2; LASS2; TMSG1; Ceramide synthase 2; CerS2; LAG1 longevity assurance homolog 2; SP260; Sphingosine N-acyltransferase CERS2; Tumor metastasis-suppressor gene 1 protein; Very-long-chain ceramide synthase CERS2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-380
  • 氨基酸序列
    MLQTLYDYFWWERLWLPVNLTWADLEDRDGRVYAKASDLYITLPLALLFLIVRYFFELYV ATPLAALLNIKEKTRLRAPPNATLEHFYLTSGKQPKQVEVELLSRQSGLSGRQVERWFRR RRNQDRPSLLKKFREASWRFTFYLIAFIAGMAVIVDKPWFYDMKKVWEGYPIQSTIPSQY WYYMIELSFYWSLLFSIASDVKRKDFKEQIIHHVATIILISFSWFANYIRAGTLIMALHD SSDYLLESAKMFNYAGWKNTCNNIFIVFAIVFIITRLVILPFWILHCTLVYPLELYPAFF GYYFFNSMMGVLQLLHIFWAYLILRMAHKFITGKLVEDERSDREETESSEGEEAAAGGGA KSRPLANGHPILNNNHRKND
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very-long-chain fatty acyl-CoA (chain length C22-C27). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively. Plays a non-redundant role in the synthesis of ceramides with very-long-chain fatty acids in kidney, liver and brain. Regulates the abundance of myelin-specific sphingolipids galactosylceramide and sulfatide that affects myelin sheath architecture and motor neuron functions.
  • 基因功能参考文献：
    1. As potential molecular markers for bladder carcinoma, both TWIST1 and LASS2 transcripts seem to play role during the tumorigenesis and development of bladder cancer. PMID: 30213291
    2. Results show that silencing of ATP6V0C in highly metastatic prostate cancer (PC) cell lines, inhibited V-ATPase activity, which coincided with the inhibition of cell migration and invasion in vitro, as well as a marked decrease in the expression of LASS2/TMSG1 probably through positive feedback. PMID: 29138865
    3. CerS2-knockdown via CRISPR-Cas9 technology in cultured colon epithelial cells impaired barrier function. PMID: 28699686
    4. Low expression of LASS2 and TGFB1 contributes to the aggressiveness and poor prognosis of hepatocellular carcinoma, and may represent a novel prognostic biomarker for hepatocellular carcinoma patients. PMID: 27581744
    5. ASGR1 can inhibit the activity of V-ATPase by interacting with LASS2, thereby suppressing the metastatic potential of hepatoma cells. PMID: 27241665
    6. These results suggest that the phosphorylation of ceramide synthases may be a key regulatory point in the control of the distribution and levels of sphingolipids of various acyl-chain lengths. PMID: 26887952
    7. Data show that 1-acylglycerol-3-phosphate O-acyltransferase 9 (AGPAT9) inhibit cell growth by regulating expression of KLF4/LASS2/V-ATPase proteins in breast cancer. PMID: 26110566
    8. these results indicate that miR-9 upregulation might be associated with malignant phenotype of bladder cancer. miR-9 promotes chemoresistance of bladder cancer cells by target LASS2. PMID: 26150338
    9. Data show that CERS2 expression was markedly different between various breast cancer cells and inversely correlated with cell invasion. PMID: 25213553
    10. silencing of TMSG1 increased V-ATPase activity, decreased extracellular pH and in turn the activation of secreted MMP-2, which ultimately promoted metastasis capacity of breast cancer cell. PMID: 25973015
    11. Results confirmed that TMSG1 is a potential metastasis suppressor gene, and suggested that the mechanism involved the induction of apoptosis and inhibition of cell proliferation via a caspase-dependent mitochondrial pathway. PMID: 25735224
    12. the vacuolar ATPase (V-ATPase) activity and extracellular hydrogen ion concentration were significantly decreased and the activity of secreted matrix metalloproteinase-2 (MMP-2) was downregulated in MCF-7 cells overexpressing LASS2/TMSG1 PMID: 25501280
    13. the inhibitory effect of the LASS2 on growth, invasion and metastasis of prostate cancer cells PMID: 24453046
    14. Co-expression of CerS2 with CerS4/CerS6 reversed the inhibitory effect of long chain ceramides on cell proliferation and the induction of apoptosis. we detected no effect on cell proliferation. PMID: 23538298
    15. expression and role of ceramide synthase-2 in the lung PMID: 23690971
    16. results contribute to the conclusion that LASS2/TMSG1 could regulate V-ATPase activity and intracellular pH through the direct interaction of its homeodomain and the C subunit of V-ATPase PMID: 22991218
    17. LASS2 expression may be correlated with the development and progression of human bladder carcinoma PMID: 21755371
    18. LASS2 is involved in chemotherapeutic outcomes and low LASS2 expression may predict chemoresistance. PMID: 22580606
    19. Silencing of LASS2 can promote invasion of prostate cancer cells in vitro through the increase of V-ATPase activity, extracellular hydrogen ion concentration and activation of secreted MMP-2. PMID: 22178826
    20. There is a nucleolar localization signal within TMSG-1. PMID: 22336162
    21. silencing of LASS2/TMSG1 can promote invasion of prostate cancer cell in vitro through increase of V-ATPase activity which accelerated tumor's invasion and metastasis, indicating that LASS2/TMSG1 is a novel tumor metastasis suppressor gene PMID: 22573553
    22. Interaction of KLF6 and Sp1, together with their binding of elements in exon 1 are critical events in initiation of transcription of the tmsg-1 gene. PMID: 21928351
    23. KLF6 and Sp1 may participate in the inducible transcriptional regulation of TMSG-1 in prostate carcinoma cells. PMID: 22169644
    24. TMSG-1 overexpression caused strong inhibition of proliferation and decreased clonogenicity of MDA-MB-231 cells, and promoted cell apoptosis. PMID: 18194600
    25. Overexpression of CerS2 resulted in partial protection from ionizing radiation-induced apoptosis PMID: 20406683
    26. CerS2 and CerS6 mRNA was significantly elevated in breast cancer tissue compared to paired normal tissue, with approximately half of the individuals showing elevated CerS2 and CerS6 mRNA. PMID: 19912991
    27. activity of CerS2 can be regulated by another bioactive sphingolipid, sphingosine 1-phosphate PMID: 18165233
    28. CerS2 down-regulation had a broad effect on ceramide homoeostasis, not just on very-long-chain ceramides. PMID: 19728861

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 组织特异性：
    Expressed in kidney, liver, brain, heart, placenta and lung.
  • 数据库链接：

    HGNC: 14076

    OMIM: 606920

    KEGG: hsa:29956

    STRING: 9606.ENSP00000271688

    UniGene: Hs.744017