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  4. Recombinant Schizosaccharomyces pombe Alkyltransferase-like protein 1 (atl1)

Recombinant Schizosaccharomyces pombe Alkyltransferase-like protein 1 (atl1)

  • 中文名称：
    Recombinant Schizosaccharomyces pombe Alkyltransferase-like protein 1(atl1),Yeast
  • 货号：
    CSB-YP892425SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Alkyltransferase-like protein 1(atl1),Yeast
  • 货号：
    CSB-EP892425SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Alkyltransferase-like protein 1(atl1),Yeast
  • 货号：
    CSB-EP892425SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Alkyltransferase-like protein 1(atl1),Yeast
  • 货号：
    CSB-BP892425SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Alkyltransferase-like protein 1(atl1),Yeast
  • 货号：
    CSB-MP892425SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    atl1
  • Uniprot No.：
  • 别名：
    atl1; SPAC1250.04cAlkyltransferase-like protein 1
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-108
  • 氨基酸序列
    MRMDEFYTKV YDAVCEIPYG KVSTYGEIAR YVGMPSYARQ VGQAMKHLHP ETHVPWHRVI NSRGTISKRD ISAGEQRQKD RLEEEGVEIY QTSLGEYKLN LPEYMWKP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in DNA damage recognition. Binds DNA containing O(6)-methylguanine and larger O(6)-alkylguanine adducts. The DNA is bent, the damaged base is rotated out of the DNA duplex into a hydrophobic binding pocket (nucleotide flipping), with Arg-39 donating a hydrogen bond to the orphaned cytosine to stabilize the extrahelical DNA conformation. This structural change in DNA presents the lesion to the nucleotide excision repair (NER) pathway. The affinity for O(6)-alkylguanine adducts increases with the size of the alkyl group. Low affinity small O(6)-alkylguanines are directed to the global genome repair pathway of NER via rhp7-rhp16 and rhp41-rhp23, while strong binding to bulky O(6)-alkylguanines stalls the transcription machinery and diverts the damage to the transcription-coupled repair pathway of NER via rhp26.
  • 基因功能参考文献：
    1. Alkyltransferase-like protein (Atl1) distinguishes alkylated guanines for DNA repair using cation-pi interactions. PMID: 23112169
    2. Data show that alkyltransferase-like 1 (Atl1)can play a pivotal role in selecting a specific nucleotide excision repair (NER) pathway, depending on the nature of the DNA modification. PMID: 22658721
    3. Atl1 may act by binding to O6-alkylguanine lesions and signalling them for processing by DNA repair pathways. PMID: 16679453
    4. Schizosaccharomyces pombe ATL structures without and with damaged DNA containing the endogenous lesion O(6)-methylguanine or cigarette-smoke-derived O(6)-4-(3-pyridyl)-4-oxobutylguanine PMID: 19516334
  • 蛋白家族：
    MGMT family, ATL subfamily
  • 数据库链接：

    KEGG: spo:SPAC1250.04c

    STRING: 4896.SPAC1250.04c.1