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  4. Recombinant Salmonella typhimurium Ethanolamine ammonia-lyase heavy chain (eutB)

Recombinant Salmonella typhimurium Ethanolamine ammonia-lyase heavy chain (eutB)

  • 货号：
    CSB-YP323088SXB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP323088SXB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP323088SXB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP323088SXB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP323088SXB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    eutB
  • Uniprot No.：
  • 别名：
    eutB; STM2458Ethanolamine ammonia-lyase heavy chain; EC 4.3.1.7; Ethanolamine ammonia-lyase large subunit
  • 种属：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-453
  • 氨基酸序列
    MKLKTTLFGN VYQFKDVKEV LAKANELRSG DVLAGVAAAS SQERVAAKQV LSEMTVADIR NNPVIAYEED CVTRLIQDDV NETAYNRIKN WSISELREYV LSDETSVDDI AFTRKGLTSE VVAAVAKICS NADLIYGGKK MPVIKKANTT IGIPGTFSCR LQPNDTRDDV QSIAAQIYEG LSFGAGDAVI GVNPVTDDVE NLTRVLDTVY GVIDKFNIPT QGCVLAHVTT QIEAIRRGAP GGLIFQSICG SEKGLKEFGV ELAMLDEARA VGAEFNRIAG ENCLYFETGQ GSALSAGANF GADQVTMEAR NYGLARHYDP FLVNTVVGFI GPEYLYNDRQ IIRAGLEDHF MGKLSGISMG CDCCYTNHAD ADQNLNENLM ILLATAGCNY IMGMPLGDDI MLNYQTTAFH DTATVRQLLN LRPSPEFERW LETMGIMANG RLTKRAGDPS LFF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the deamination of various vicinal amino-alcohols to oxo compounds (Probable). It is spontaneously inactivated by its substrate and reactivated by EutA. May play a role in BMC assembly or maintenance (Probable).; Expression of the eut operon allows this bacteria to use ethanolamine (EA) as a carbon, nitrogen and energy source. It relies on cobalamin (vitamin B12) both as a cofactor for the ethanolamine ammonia-lyase activity and to induce the operon. EA enhances bacterial survival in macrophages in a concentration-dependent manner, suggesting it is an important nutrient during infection.
  • 基因功能参考文献：
    1. EutB from coenzyme B12-dependent ethanolamine ammonia-lyase has a beta8alpha8, TIM-barrel fold and radical catalytic site structural features PMID: 16688781
    2. positive charge at the residue 160 side chain terminus is required for proper folding of EutB PMID: 18444665
  • 亚细胞定位：
    Bacterial microcompartment.
  • 数据库链接：

    KEGG: stm:STM2458

    STRING: 99287.STM2458