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  4. Recombinant Saccharomyces cerevisiae SUMO-conjugating enzyme UBC9 (UBC9)

Recombinant Saccharomyces cerevisiae SUMO-conjugating enzyme UBC9 (UBC9)

  • 货号：
    CSB-YP025457SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025457SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025457SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025457SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025457SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    UBC9
  • Uniprot No.：
  • 别名：
    UBC9; YDL064W; SUMO-conjugating enzyme UBC9; EC 2.3.2.-; RING-type E3 SUMO transferase UBC9; Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-157
  • 氨基酸序列
    SSLCLQRLQ EERKKWRKDH PFGFYAKPVK KADGSMDLQK WEAGIPGKEG TNWAGGVYPI TVEYPNEYPS KPPKVKFPAG FYHPNVYPSG TICLSILNED QDWRPAITLK QIVLGVQDLL DSPNPNSPAQ EPAWRSFSRN KAEYDKKVLL QAKQYSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2).
  • 基因功能参考文献：
    1. Ubc9 sumoylation is a key event for meiotic synaptonemal complex formation. SUMO Ubc9 enhances catalysis by binding and orienting donor SUMO charged Ubc9. PMID: 23644018
    2. These findings establish a functional interaction between N-terminal and substrate-binding domains of Ubc9 and distinguish the activities of E3 ligases Siz1 and Siz2 in regulating cellular responses to genotoxic stress. PMID: 16782883
    3. analysis of a SUMO-binding-motif mimic bound to Smt3p-Ubc9p PMID: 17475278
    4. The crystal structure of sumoylated Ubc9 demonstrates how the newly created binding interface can provide a gain in affinity otherwise provided by E3 ligases. PMID: 18691969
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 数据库链接：

    KEGG: sce:YDL064W

    STRING: 4932.YDL064W