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  4. Recombinant Saccharomyces cerevisiae Protein TEM1 (TEM1)

Recombinant Saccharomyces cerevisiae Protein TEM1 (TEM1)

  • 中文名称：
    酿酒酵母TEM1重组蛋白
  • 货号：
    CSB-YP340039SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母TEM1重组蛋白
  • 货号：
    CSB-EP340039SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母TEM1重组蛋白
  • 货号：
    CSB-EP340039SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母TEM1重组蛋白
  • 货号：
    CSB-BP340039SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母TEM1重组蛋白
  • 货号：
    CSB-MP340039SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TEM1
  • Uniprot No.：
  • 别名：
    TEM1; YML064C; Protein TEM1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-245
  • 氨基酸序列
    MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT QTLGVNFLKR KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF DLTRPETLSS IKEWYRQAYG LNDSAIPILV GTKYDLLIDL DPEYQEQISR TSMKYAQVMN APLIFCSTAK SINIQKIFKI ALAKIFNLTL TIPEINEIGD PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    GTP-binding protein involved in termination of M phase. May play a role in triggering the degradation of G2 cyclin to inactivate M-phase promoting factor at the termination of mitosis. Acts upstream of CDC15 kinase and may be required to activate the CDC15 protein kinase pathway.
  • 基因功能参考文献：
    1. Tem1 residence at SPBs depends on its nucleotide state and, importantly, asymmetry of the Bub2-Bfa1-Tem1 complex does not promote mitotic exit but rather controls spindle positioning. PMID: 26507466
    2. asymmetry of the Bub2/Bfa1/Tem1 complex is crucial to control Kar9 distribution and spindle positioning during mitosis. PMID: 25658911
    3. Bfa1 localization at spindle pole bodies (SPBs) is independent of Tem1; TEM1 neither alters Bfa1 accumulation at daughter-directed SPB in normal cells nor prevents the localization of Bfa1 at both SPBs in cells with misaligned spindles. PMID: 19948498
  • 数据库链接：

    KEGG: sce:YML064C

    STRING: 4932.YML064C