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  4. Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor 18 (PRP18)

Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor 18 (PRP18)

  • 货号：
    CSB-YP018760SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018760SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018760SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018760SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018760SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRP18
  • Uniprot No.：
  • 别名：
    PRP18; YGR006W; Pre-mRNA-splicing factor 18
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-251
  • 氨基酸序列
    MDLDLASILK GEISKKKKEL ANSKGVQPPC TEKFQPHESA NIDETPRQVE QESTDEENLS DNQSDDIRTT ISKLENRPER IQEAIAQDKT ISVIIDPSQI GSTEGKPLLS MKCNLYIHEI LSRWKASLEA YHPELFLDTK KALFPLLLQL RRNQLAPDLL ISLATVLYHL QQPKEINLAV QSYMKLSIGN VAWPIGVTSV GIHARSAHSK IQGGRNAANI MIDERTRLWI TSIKRLITFE EWYTSNHDSL A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the U4/U5/U6 snRNP, binding principally to the u5 snRNP. It is not absolutely required for the second step of pre-mRNA splicing at low temperatures but is required at higher temperatures. It may stabilize a particular conformation of the U5 snRNP or orient the U5 snRNP within the U4/U5/U6 snRNP or within the spliceosome.
  • 基因功能参考文献：
    1. structure along with sequence comparisons among SRA1p orthologs and against authentic RNA recognition motif (RRM) proteins indicates that it is not an RRM domain but rather an all-helical protein with a fold more similar to the PRP18 splicing factor PMID: 24486611
    2. Prp18 acts in concert with U5 snRNA during the second step of pre-mRNA splicing. PMID: 15743809
    3. The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing. PMID: 17504938
    4. Arginine1753 of Prp8 collaborates with Prp18 to stabilize U5 snRNA/exon contacts that are important for the second transesterification step of pre-mRNA splicing. PMID: 17626844
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    PRP18 family
  • 数据库链接：

    KEGG: sce:YGR006W

    STRING: 4932.YGR006W