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  4. Recombinant Rat Amiloride-sensitive sodium channel subunit beta (Scnn1b), partial

Recombinant Rat Amiloride-sensitive sodium channel subunit beta (Scnn1b), partial

  • 货号：
    CSB-YP020849RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP020849RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP020849RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP020849RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP020849RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Scnn1b
  • Uniprot No.：
  • 别名：
    Scnn1b; Amiloride-sensitive sodium channel subunit beta; Beta-NaCH; Epithelial Na(+) channel subunit beta; Beta-ENaC; Nonvoltage-gated sodium channel 1 subunit beta; SCNEB
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
  • 基因功能参考文献：
    1. deltabetagamma-ENaC is inhibited by CFTR but activated by cyclic AMP. PMID: 27941075
    2. Decreased expression of Scnn1b/g and activation of ENaC could be involved in attenuation of the aortic baroreceptor sensitivity in congestive heart failure. PMID: 26297031
    3. level of ENaC-alpha expression decreased 0.50-fold and 0.55-fold at 6 and 12 h after an injection of LPS into the middle ear cavity PMID: 22830999
    4. ENaC-gamma and -beta play an important role as mechanosensitive ion channels initiating pressure-induced myogenic responses in rat posterior cerebral arteries. PMID: 22090066
    5. Data indicate that functional ENaC (subunits Scnn1a, Scnn1b, and Scnn1g) may affect firing patterns of magnocellular cells (i.e., large neurons) in supraoptic nucleus and paraventricular nucleus and thus control secretion of vasopressin and oxytocin. PMID: 22045317
    6. Persistent high expression of alphaENaC and increase in apical localization of beta and gammaENaC may contribute to greater retention of sodium in salt-resistant rats on a high-salt diet. PMID: 21423289
    7. Each of the beta-subunit isoforms (beta1-beta4) of sodium channel was expressed in neonatal cardiac cells. PMID: 19426735
    8. elucidate key aspects of ENaC trafficking and turnover rates in mammalian kidney epithelial cells PMID: 11773057
    9. Interactions of beta and gamma ENaC with Nedd4 can be facilitated by an ERK-mediated phosphorylation PMID: 11805112
    10. hypoxia-induced inhibition of amiloride-sensitive sodium channel activity is mediated by decreased apical expression of ENaC subunits PMID: 12372821
    11. inhibitory effect of cAMP-activated CFTR on ENaC is due to a decrease in channel open probability. PMID: 12548398
    12. mutants causing Liddle's syndrome retain ability to respond to aldosterone and vasopressin PMID: 12759227
    13. ATP regulation of ENaC may be involved in the fine-tuning of ENaC activity under physiologic and pathophysiologic conditions PMID: 12876281
    14. segment-specific downregulation of beta-ENaC and gamma-ENaC in the cortical and outer medullary collecting duct, chief sites for collecting duct sodium reabsorption, in rats with a lithium-induced increase in fractional excretion of sodium. PMID: 12928314
    15. Modulation of ENaC Po by endogenous epithelial serine proteases is potentially important regulator of epithelial Na+ transport, distinct from regulation achieved by hormone-induced plasma membrane insertion of channels. PMID: 12967915
    16. TNF-alpha decreased the expression of the alpha-, beta-, and gamma-subunits of epithelial sodium channel (ENaC) mRNA to 36, 43, and 16% of the controls after 24-h treatment and reduced to 50% the amount of alpha-ENaC protein in lung epithelium PMID: 14514522
    17. The absence of stimulation of ENaCalpha mRNA expression in collecting ducts suggests the physiological significance of ENaCbeta and gamma mRNA for acute regulation by vasopressin. PMID: 14567502
    18. A heterooligomer composed of alpha-, beta-, and gamma-ENaC may be the molecular basis of the native channels, which are responsible for amiloride-sensitive electrogenic Na+ absorption in rat rectal colon. PMID: 14576089
    19. Results suggest that there are different translational efficiencies for epithelial sodium channel subunits alpha, beta and gamma, and that perinatal processes globally modulate lung mRNA translation. PMID: 14672917
    20. the increased or sustained expression of ENaC subunits combined with increased apical targeting in the distal tubule, connecting tubule, and collecting duct are likely to play a role in the sodium retention associated with PAN-induced nephrotic syndrome PMID: 15075188
    21. inhibitory effect of [Cl-]i occurred regardless of the presence or absence of net Cl- transport. PMID: 15161604
    22. Alpha-, beta- and gamma-ENaC were extensively labeled in the inner ear. Functional ENaC in the labyrinth may work in concert with other Na+ and K+ transport molecules to regulate endolymph and to maintain homeostasis in the inner ear. PMID: 15219314
    23. expressed in vascular smooth muscle cells; data suggest that DEG/ENaC proteins are required for vessel responses to pressure and are consistent with the evolutionary conservation of mechanosensory function of DEG/ENaC proteins PMID: 15381679
    24. Steady state renal mRNA of ENaC alpha, beta and gamma were unchanged in liver cirrhosis. PMID: 15956070
    25. ENaC mRNA and protein expression are not increased in the early phase of unilateral puromycin aminonucleoside-induced nephrotic syndrome PMID: 16020936
    26. A role is confirmed for the carboxyl terminus of betaENaC for chloride ion-dependent inhibition of the Na+ channel, which may be part of a complex regulation of ENaC by cystic fibrosis transmembrane conductance regulator. PMID: 16027156
    27. the binding of active Nedd4-2 to ENaC is a crucial step in the mechanism of ENaC inhibition by progesterone PMID: 16172119
    28. ENaC stimulation by cAMP involves putative ERK phosphorylation sites in the C termini of the channel's beta- and gamma-subunit PMID: 16476738
    29. Salt restriction increased the abundance of the beta-subunit of ENaC and produced an endoglycosidase H (Endo H)-resistant pool of this subunit. PMID: 16554417
    30. fully glycosylated form of beta-ENaC in Na-depleted animals was observed mainly in outer & inner medulla. findings suggest that capability of hormone-regulated, channel-mediated Na reabsorption by the nephron extends into outer medullary collecting duct. PMID: 17200158
    31. Results suggest that the ENaC PY motifs (and Nedd4-2) primarily regulate channel internalization but also affect cAMP-dependent replenishment, providing important insight into the Liddle syndrome defects. PMID: 17605762
    32. Epidermal growth factor inhibits sodium absorption via an ERK1/2-mediated down-regulation of Scnnb1. PMID: 17724164
    33. ENaC beta is functionally related to regulatory volume increase and hypertonicity-induced cation channel activation in rat hepatocytes PMID: 17926064
    34. Dietary sodium chloride induced translocation of Scnn1b from intracellular regions to the cell membrane of vascular smooth muscle cells. PMID: 18024548
    35. Expressed at the apical membrane of the principal cells in rats; a low-Na diet or aldosterone perfusion increases expression. PMID: 18504317
    36. support a model in which protons modulate ENaC gating by relieving Na(+) self-inhibition PMID: 18990692
    37. Altered renal expression of Scnn1b in protein-deprived rats. PMID: 19202345
    38. ENaCbeta is a key mechanotransducing channel in the periodontal Ruffini endings. Probably, the terminal Schwann cells together with the axon terminals regulate mechanotransduction in the periodontal endings PMID: 19420735

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  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family, SCNN1B subfamily
  • 组织特异性：
    Expressed in lung and epididymis. In the caput region of the epididymis, expressed at the luminal and basolateral surfaces of the ducts and in the smooth muscle coat. In the caudal region of the epididymis, expressed along the luminal border but not conti
  • 数据库链接：

    KEGG: rno:24767

    STRING: 10116.ENSRNOP00000063755

    UniGene: Rn.9807