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  4. Recombinant Mouse Sialidase-3 (Neu3)

Recombinant Mouse Sialidase-3 (Neu3)

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  • 中文名称：
    小鼠Neu3重组蛋白
  • 货号：
    CSB-EP872896MO
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Neu3
  • Uniprot No.：
  • 别名：
    Neu3Sialidase-3; EC 3.2.1.18; Ganglioside sialidase; Membrane sialidase; N-acetyl-alpha-neuraminidase 3
  • 种属：
    Mus musculus(Mouse)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    52.9 kDa
  • 表达区域：
    1-418aa
  • 氨基酸序列
    MEEVPPYSLSSTLFQQEEQSGVTYRIPALLYLPPTHTFLAFAEKRTSVRDEDAACLVLRRGLMKGRSVQWGPQRLLMEATLPGHRTMNPCPVWEKNTGRVYLFFICVRGHVTERCQIVWGKNAARLCFLCSEDAGCSWGEVKDLTEEVIGSEVKRWATFAVGPGHGIQLHSGRLIIPAYAYYVSRWFLCFACSVKPHSLMIYSDDFGVTWHHGKFIEPQVTGECQVAEVAGTAGNPVLYCSARTPSRFRAEAFSTDSGGCFQKPTLNPQLHEPRTGCQGSVVSFRPLKMPNTYQDSIGKGAPATQKCPLLDSPLEVEKGAETPSATWLLYSHPTSKRKRINLGIYYNRNPLEVNCWSRPWILNRGPSGYSDLAVVEEQDLVACLFECGEKNEYERIDFCLFSDHEVLSCEDCTSPSSD
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes.
  • 基因功能参考文献：
    1. the expression of neuraminidases in induced regulatory T (iTreg) cells was investigated in comparison with that in other T-cell subsets and concluded that Neu3 contributes to the differentiation of iTreg cells by upregulation of FoxP3. PMID: 29271120
    2. radiographs revealed abnormalities in the skeletal bones of the Hexa(-/-)Neu3(-/-) mice PMID: 28974375
    3. These results suggest that NEU3 activity on exosome glycans enhances the dynamic biological behavior of these small extracellular vesicles by modifying the negative charge and steric hindrance of their glycocalyx, and that the presence of NEU3 on the exosomal surface could represent a useful marker for the detection of these nanovesicles PMID: 29039925
    4. These results provide the first in vivo evidence that neuraminidases 3 and 4 have important roles in CNS function by catabolizing gangliosides and preventing their storage in lipofuscin bodies. PMID: 28442549
    5. findings implicate NEU3 as a potential biomarker of insulin sensitivity, and provide novel mechanistic insight into the regulation of NEU3 expression. PMID: 26022181
    6. NEU3 regulates the detergent resistant membrane ganglioside content. PMID: 24925219
    7. NEU3 up-regulation stimulates the EGF receptor signaling pathway, which in turn activates the hypoxia-inducible factor (HIF-1alpha), resulting in a final increase of cell survival and proliferation. PMID: 23209287
    8. NEU3 plays an important role in inflammation-dependent tumor development PMID: 22815940
    9. Data indicate that sialidases Neu1 and Neu3 are present on sperm, and their activity is required for capacitation and zona pellucida binding. PMID: 22989879
    10. The work further supports the central role of NEU3 as a key modulator in skeletal muscle differentiation, particularly in the myoblast fusion step. PMID: 22552967
    11. It exists in membrane microdomains and regulates signal transductions. (review) PMID: 21089368
    12. NEU3 participates in the control of insulin signaling via modulation of gangliosides and interaction with Grb2 PMID: 12730204
    13. MmNEU3 is able to hydrolyze ganglioside substrates in intact living cells at a neutral pH, mainly through cell-to-cell interactions PMID: 14970224
    14. NEU3 is able to mobilize to membrane ruffles in response to growth stimuli and activate the Rac-1 signaling by co-localization with Rac-1, leading to increased cell motility. PMID: 16765317
    15. Reduction of 70% of the plasma membrane-associated sialidase Neu3 activity caused neurite elongation in Neuro2a murine neuroblastoma cells. PMID: 17176265
    16. findings suggest that the Neu3 gene represents a physiological modulator of VSMC responses that may contribute to plaque instability in atherosclerosis. PMID: 17382908
    17. The decrease of GD1a in COS7 cell membrane upon MmNEU3 over-expression is likely to be instrumental to Newcastle Disease Virus reduced infection. PMID: 18155174
    18. NEU3 sialidase strictly modulates GM3 levels in skeletal myoblasts C2C12 thus favoring their differentiation and protecting them from apoptosis PMID: 18945680
    19. Plasma membrane-associated sialidase (Neu3) promotes formation of colonic aberrant crypt foci in azoxymethane-treated transgenic mice. PMID: 19215228

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Membrane, caveola. Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Glycosyl hydrolase 33 family
  • 组织特异性：
    Expressed in heart, brain and cerebral cortex.
  • 数据库链接：

    KEGG: mmu:50877

    STRING: 10090.ENSMUSP00000045222

    UniGene: Mm.103703