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  4. Recombinant Methanosphaera stadtmanae Flap endonuclease 1 (fen)

Recombinant Methanosphaera stadtmanae Flap endonuclease 1 (fen)

  • 中文名称：
    Recombinant Methanosphaera stadtmanae Flap endonuclease 1(fen),Yeast
  • 货号：
    CSB-YP646725MAAR
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Methanosphaera stadtmanae Flap endonuclease 1(fen),Yeast
  • 货号：
    CSB-EP646725MAAR
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Methanosphaera stadtmanae Flap endonuclease 1(fen),Yeast
  • 货号：
    CSB-EP646725MAAR-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Methanosphaera stadtmanae Flap endonuclease 1(fen),Yeast
  • 货号：
    CSB-BP646725MAAR
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Methanosphaera stadtmanae Flap endonuclease 1(fen),Yeast
  • 货号：
    CSB-MP646725MAAR
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    fen
  • Uniprot No.：
  • 别名：
    fen; Msp_1086Flap endonuclease 1; FEN-1; EC 3.1.-.-; Flap structure-specific endonuclease 1
  • 种属：
    Methanosphaera stadtmanae (strain DSM 3091)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-328
  • 氨基酸序列
    MGVKFKDITN PEPIEMKELE GKILTVDASN VIYKFLSSMR QTDGTPLRDL NGHITSHLNG IMFQTSTLIE KDIKPVYVFD GKAPDLKKET QEERINIKKE SEKKYLEAKE VGDVVAARKY AARTTHLNKE IIKSSKKLLD LMGIPYVQAR TEGEAQASYM VSQNDAWAVV SQDYDCLQFG ATRMIRNLKL SKSNSKNLEL ISLEKTLKEL NLTREQLVDV AMLVGTDFNK GVYGIGAKKG IKLIHKYGTL EKALESLNET MEVDAELIRE IFLNPNVVHN YTIEFKRPKK SQLLDFLCGE HDFDERRTIS AIKKLQAKTA QSSLEDWF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
  • 蛋白家族：
    XPG/RAD2 endonuclease family, FEN1 subfamily
  • 数据库链接：

    KEGG: mst:Msp_1086

    STRING: 339860.Msp_1086