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  4. Recombinant Human Protamine-2 (PRM2)

Recombinant Human Protamine-2 (PRM2)

  • 货号：
    CSB-YP018729HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018729HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018729HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018729HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018729HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRM2
  • Uniprot No.：
  • 别名：
    PRM2; Protamine-2; Sperm histone P2; Sperm protamine P2) [Cleaved into: Basic nuclear protein HPI1; Basic nuclear protein HPI2; Basic nuclear protein HPS1; Basic nuclear protein HPS2; Sperm histone HP4; Sperm protamine P4); Sperm histone HP2; Sperm protamine P2; P2'); Sperm histone HP3; P2''; Sperm protamine P3)]
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-102
  • 氨基酸序列
    VRYRVRSLS ERSHEVYRQQ LHGQEQGHHG QEEQGLSPEH VEVYERTHGQ SHYRRRHCSR RRLHRIHRRQ HRSCRRRKRR SCRHRRRHRR GCRTRKRTCR RH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.
  • 基因功能参考文献：
    1. s examined, for the first time in a Turkish population, the association between protamine gene alleles (PRM1 c.-190C>A, PRM1 c.197G>T, and PRM2 c.248C>T), and YBX2 (c.187T>C and c.1095 + 16A>G) and male infertility. PMID: 29227750
    2. PRM2 gene expression was significantly decreased in immature sperm extracted from the fertile and infertile groups. Caspase 9 activity was significantly increased in immature sperm extracted from both groups. PMID: 26392304
    3. The expressed human protamine2 in two fast-growing cell systems, E. coli and HeLa cells. The protamine2 expression significantly attenuated cell proliferation when compared with control cells. PMID: 25649960
    4. Data indicate changed ecpression levels of PRM2, HSP90 and WNT5A in male of couples experiencing idiopathic recurrent miscarriages. PMID: 23271023
    5. Single nuclotide polymorphisms in PRM2 gene is associated with male infertility. PMID: 22104739
    6. analyse the presence of DAZ, RBMY1, USP9Y, protamine-2, SRY and actin messenger RNA (mRNA) in testicular cells of men suffering from idiopathic azoospermia. PMID: 11869379
    7. significantly enhanced evolutionary rates in the hominid lineage PMID: 12524352
    8. Altered protamine P1/P2 ratio effects fertilization rate and embryo quality which subsequently may affect implantation and pregnancy outcome. PMID: 15670415
    9. levels of pre-P2 may provide clues into pathogenesis of infertility. increased proportion of pre-P2 in some men with increased P1/P2 ratio suggests involvement of pre-P2 processing & link between deficient protamine processing & decreased DNA integrity. PMID: 16632464
    10. Novel polycythemia vera (PV)-associated tumor antigen PV13 (protamine 2) elicits IgG antibody reactions in a subset of PV patients but not in healthy donors, suggesting that it is an authentic tumor antigens. PMID: 17113348
    11. The PRM2 contributes not only to successful fertilization, but may has an important impact in development of preimplantation embryos. PMID: 18292840
    12. A reduction in contents of PRM2 transcript in spermatozoa may be linked with asthenozoospermia. PMID: 18292846

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Protamine P2 family
  • 组织特异性：
    Testis.
  • 数据库链接：

    HGNC: 9448

    OMIM: 182882

    KEGG: hsa:5620

    STRING: 9606.ENSP00000241808

    UniGene: Hs.2324