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  4. Recombinant Escherichia coli RNA polymerase sigma factor rpoS (rpoS)

Recombinant Escherichia coli RNA polymerase sigma factor rpoS (rpoS)

  • 货号：
    CSB-YP319548ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP319548ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP319548ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP319548ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpoS
  • Uniprot No.：
  • 别名：
    rpoS; appR; katF; nur; otsX; sigS; b2741; JW5437; RNA polymerase sigma factor RpoS; Sigma S; Sigma-38
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-330
  • 氨基酸序列
    MSQNTLKVHD LNEDAEFDEN GVEVFDEKAL VEQEPSDNDL AEEELLSQGA TQRVLDATQL YLGEIGYSPL LTAEEEVYFA RRALRGDVAS RRRMIESNLR LVVKIARRYG NRGLALLDLI EEGNLGLIRA VEKFDPERGF RFSTYATWWI RQTIERAIMN QTRTIRLPIH IVKELNVYLR TARELSHKLD HEPSAEEIAE QLDKPVDDVS RMLRLNERIT SVDTPLGGDS EKALLDILAD EKENGPEDTT QDDDMKQSIV KWLFELNAKQ REVLARRFGL LGYEAATLED VGREIGLTRE RVRQIQVEGL RRLREILQTQ GLNIEALFRE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management.; Protects stationary phase cells from killing induced by endoribonuclease MazF.
  • 基因功能参考文献：
    1. RpoS contributes to the resistance of E. coli to protozoan predation and that RpoS is crucial for the increased fitness of soil-persistent E. coli against predation and reduced moisture in soil. PMID: 28967947
    2. bolA plays a major role in the presence and absence of rpoS in attachment to the stainless steel and silicone surfaces. PMID: 27842452
    3. RNA polymerase is pausing during initial transcription. PMID: 27618490
    4. Our study indicates that the RNA chaperone Hfq contributes to the persistent infection of E. coli by maintaining the expression of bacterial genes, including one coding for sigma(38), that help bacteria evade host immunity. PMID: 27357148
    5. A promoter's responsiveness to indirect regulation by sigma factor competition is determined by the sequence-dependent kinetics of the rate limiting steps of transcription initiation. PMID: 27452766
    6. results suggested that the determinant for sigma38-dependent promoter lies in the promoter upstream sequence PMID: 25845539
    7. the bacterial cell responds to lowered levels of acetyl-CoA by inducing RpoS, allowing reprogramming of E. coli metabolism. PMID: 25847996
    8. It was thus verified that the reinforcement of csgA gene in the DeltarpoS cells induced the enhanced colonization on the solid surfaces with the increased flagellum and curli expressions. PMID: 25252647
    9. Environmental stresses resulting in reduction in growth rate stimulate the expression of the rpoS gene. [review] PMID: 24596257
    10. All the relocated AAN binding sites restored tight Hfq binding in vitro, but only insertion at the natural position restored Hfq-dependent sRNA annealing in vitro and sRNA regulation of rpoS translation in vivo. PMID: 24051417
    11. Dependence on MiaA may therefore provide yet another way for RpoS levels to respond to growth conditions. PMID: 24296670
    12. Exposure to H2O2 during extreme-acid treatment increased the death rate slightly for W3110 and to a greater extent for the rpoS deletion strain. PMID: 23520457
    13. We finally demonstrate that the RpoS general stress regulator prevents oxidative stress-mediated DNA damage formation in E. coli. PMID: 23613664
    14. These results suggest that rpoS does have an influence on both biofilm formation and survival of Escherichia coli O157:H7 and that the advantage conferred by rpoS is contingent on the environmental conditions. PMID: 23001657
    15. Peroxide resistance was greater (P<0.05) in biofilm cells than in planktonic cells, and full resistance required rpoS but not oxyR. PMID: 22700652
    16. findings demonstrate that naturally occurring rare codons in rpoS contribute to protecting the rpoS transcript against RNase E-mediated degradation PMID: 21788735
    17. The inactivation of rpoS systematically reduced transformation frequency, while over-expressing rpoS increased plasmid transformation. PMID: 22438941
    18. These data suggest that cellular ATP levels directly control RpoS stability. PMID: 22426532
    19. We propose that RpoS-regulated functions are responsible for the increase in membrane resilience as cells enter stationary phase and that this plays a major role in the development of pressure resistance. PMID: 21705547
    20. OxyS represses rpoS mRNA translation by sequestering Hfq rather than binding to rpoS mRNA PMID: 21889623
    21. Expression of the flagellar sigma(F) factor (FliA), another sigma(70) family protein, is controlled positively by RpoN but negatively by RpoS. PMID: 21219458
    22. K(+) is responsible for influencing the binding of sigma(70) and sigma(S) to core RNA polymerase. PMID: 21143318
    23. Increased mutation frequency is observed in redox-impaired Escherichia coli due to RelA- and RpoS-mediated repression of DNA repair. PMID: 20581184
    24. Found that the level of the small regulatory RNA RprA, which is involved in the promotion of rpoS translation, is higher in pgsA3 cells than in pgsA(+) cells. PMID: 20455949
    25. The results identify a second DNA polymerase contributing to stress-induced mutagenesis and show that RpoS promotes mutagenesis by more than the simple upregulation of dinB. PMID: 20639336
    26. sigma(70) and sigma(38) dependent promoters in all glycolytic genes and operons could allow a differential transcription of these central metabolism genes by both sigma subunits as an adaptation response to carbon limitation PMID: 19838295
    27. Polyamines, in particular putrescine, are involved in regulation of the rpoS expression by concentration-dependent stimulation at the levels of translation and stability of the protein sigmaS PMID: 15377267
    28. RpoS proteolysis is regulated upon carbon starvation by a mechanism that does not require the SprE (RssB) response regulator phosphorylation site. PMID: 15489452
    29. low shear simulated microgravity rendered rpoS less stable in exponential phase and increased rpoS mRNA translational efficiency PMID: 15576768
    30. identification of genes that require RpoS for expression PMID: 15576800
    31. required for activation of gab operon in stress induced pathway mediated by loss of LPS inner core PMID: 15576807
    32. Esigma(s) favours the presence of a distal UP-element half-site, and at the same time is unable to take advantage of a proximal half-site or a full UP-element. Esigma70, however, exhibits the opposite preference. PMID: 15612932
    33. we demonstrate that RpoS levels following ammonia starvation are only slightly increased compared to growing cells and are 10-fold below the levels observed under glucose or phosphate limitation. PMID: 15629914
    34. Genome-wide expression profiling data presented here indicate that up to 10% of the E. coli genes are under direct or indirect control of sigmaS. PMID: 15716429
    35. An increased level of alternative sigma factor RpoS partially suppresses drug hypersensitivity associated with inactivation of the multidrug resistance pump AcrAB in Escherichia coli. PMID: 15808939
    36. mediates differential growth-dependent expressions of the tktA and tktB genes PMID: 15968503
    37. findings show that 24 nucleotides of the rpoS ribosome-binding site (RBS) are necessary and sufficient for a large part of the increase in rpoS translation as cells grow to stationary phase PMID: 16237004
    38. Inactivation of IHF in vivo abolishes the sigma (S)-dependent transcription initiation of the pst operon. PMID: 16404567
    39. The data suggest that an ensemble of -35 region elements exists at sigma38 promoters and these can help mediate responsiveness to physiological challenges through interactions involving region 4 of the sigma38 protein. PMID: 16420371
    40. analysis of rpoS mutations in Escherichia coli populations PMID: 16489226
    41. Analysis of the stability of sigmaS and the levels of RssB indicate that the absence of PAPI enhances RssB-mediated sigmaS proteolysis specifically in starved cells. PMID: 16556229
    42. Regulated interplay between the mobile modules of the beta' and the sigma subunits of the RNA Polymerase appears to be necessary for stable Open promoter Complex formation. PMID: 16725156
    43. Pfs transcription is shown to rely on both sigma 70 and sigma 38 (rpoS), and the latter is subject to induction that increases pfs expression. PMID: 16950920
    44. The regulatory protein Crl increases the activity of RpoS by direct interaction with the RpoS holoenzyme. PMID: 17224607
    45. study elucidates rules governing RpoS selectivity in presence of a class II activator, provide insight into transcriptional activation by Fis from this position & clarify, why the proP promoter is activated during a short time when Fis & RpoS are present PMID: 17302803
    46. the accumulation and activation of sigma(S) upon carbon starvation, which are linked to alterations in both ribosomal fidelity and efficiency. PMID: 17403784
    47. Results suggest that the regulatory RNA SsrA may indirectly improve RpoS translation by limiting ribosome stalling and depletion of some component of the translation machinery. PMID: 17449615
    48. cell use of ppGpp to mediate a variety of starvation responses operates in part by modulating sigma(S) levels PMID: 17640895
    49. This study presented evidence that the CsgD protein, a transcription regulator involved in biofilm formation in Escherichia coli, modulates the expression of the rpoS (sigma(S)) regulon. PMID: 17873038
    50. Real-time PCR (RT-PCR) analysis confirmed the important role of RpoS in the PTS- strains and allowed the identification of 19 genes including almost all the glycolytic genes, not previously reported, to be at least partially dependent on RpoS. PMID: 17938565

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sigma-70 factor family, RpoS subfamily
  • 数据库链接：

    KEGG: ecj:JW5437

    STRING: 316385.ECDH10B_2909