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  4. Recombinant Escherichia coli Polyribonucleotide nucleotidyltransferase (pnp)

Recombinant Escherichia coli Polyribonucleotide nucleotidyltransferase (pnp)

  • 中文名称：
    大肠杆菌pnp重组蛋白
  • 货号：
    CSB-YP361454ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌pnp重组蛋白
  • 货号：
    CSB-EP361454ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌pnp重组蛋白
  • 货号：
    CSB-EP361454ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌pnp重组蛋白
  • 货号：
    CSB-BP361454ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌pnp重组蛋白
  • 货号：
    CSB-MP361454ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pnp
  • Uniprot No.：
  • 别名：
    pnp; b3164; JW5851; Polyribonucleotide nucleotidyltransferase; EC 2.7.7.8; Polynucleotide phosphorylase; PNPase
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-711
  • 氨基酸序列
    MLNPIVRKFQ YGQHTVTLET GMMARQATAA VMVSMDDTAV FVTVVGQKKA KPGQDFFPLT VNYQERTYAA GRIPGSFFRR EGRPSEGETL IARLIDRPIR PLFPEGFVNE VQVIATVVSV NPQVNPDIVA MIGASAALSL SGIPFNGPIG AARVGYINDQ YVLNPTQDEL KESKLDLVVA GTEAAVLMVE SEAQLLSEDQ MLGAVVFGHE QQQVVIQNIN ELVKEAGKPR WDWQPEPVNE ALNARVAALA EARLSDAYRI TDKQERYAQV DVIKSETIAT LLAEDETLDE NELGEILHAI EKNVVRSRVL AGEPRIDGRE KDMIRGLDVR TGVLPRTHGS ALFTRGETQA LVTATLGTAR DAQVLDELMG ERTDTFLFHY NFPPYSVGET GMVGSPKRRE IGHGRLAKRG VLAVMPDMDK FPYTVRVVSE ITESNGSSSM ASVCGASLAL MDAGVPIKAA VAGIAMGLVK EGDNYVVLSD ILGDEDHLGD MDFKVAGSRD GISALQMDIK IEGITKEIMQ VALNQAKGAR LHILGVMEQA INAPRGDISE FAPRIHTIKI NPDKIKDVIG KGGSVIRALT EETGTTIEIE DDGTVKIAAT DGEKAKHAIR RIEEITAEIE VGRVYTGKVT RIVDFGAFVA IGGGKEGLVH ISQIADKRVE KVTDYLQMGQ EVPVKVLEVD RQGRIRLSIK EATEQSQPAA APEAPAAEQG E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction. Also involved, along with RNase II, in tRNA processing. RNases II and R contribute to rRNA degradation during starvation, while RNase R and PNPase are the major contributors to quality control of rRNA during steady state growth.
  • 基因功能参考文献：
    1. Inhibition of homologous PNPase by citrate may represent an evolutionarily conserved communicative link between RNA degradation and central metabolism. PMID: 28334892
    2. PNPase and RNase PH facilitate the stability and regulatory function of the sRNAs RyhB, CyaR, and MicA during exponential growth. PMID: 27698082
    3. A conserved loop in polynucleotide phosphorylase (PNPase) is essential for both RNA and ADP/phosphate binding. PMID: 24075876
    4. These results demonstrate that polynucleotide phosphorylase is necessary to maintain bacterial cells in the planktonic mode through down-regulation of pgaABCD expression and PNAG production. PMID: 23171129
    5. S1 and KH domains of pnp determine the efficiency of RNA binding and autoregulation. PMID: 23457244
    6. Spontaneous mutations resulting from replication errors, which are normally repaired by the mismatch repair (MMR) system, are sharply reduced in a polynucleotide phosphorylase-deficient Escherichia coli strain. PMID: 22904280
    7. Absence of PNPase decreases the survival of cells exposed to UV radiation. PMID: 22322961
    8. Role of polynucleotide phosphorylase in sRNA function in Escherichia coli PMID: 21527671
    9. Polynucleotide phosphorylase activity may be modulated by metabolites in Escherichia coli PMID: 21324911
    10. examined roles of the conserved S1 and KH RNA binding motifs in PNPase; propose that the S1 domain, possibly arrayed with the KH domain, forms an RNA binding surface that facilitates substrate recognition and thus indirectly potentiates product release PMID: 16237005
    11. results indicate that two clones of PNPase including residues 158-262 and residues 473-577 contain interaction sites for RNase E within a betabetaalphabetabetaalpha domain configuration PMID: 16483707
    12. suppression of Rho-dependent transcription termination within pnp and its restoration by PNPase is an autogenous regulatory circuit that modulates pnp expression during cold acclimation PMID: 17384964
    13. new role for PNPase in the degradation of small noncoding RNAs PMID: 18203924
    14. The constricted central channel and the basic-charged residues in the channel necks of PNPase play crucial roles in trapping RNA for processive exonucleolytic degradation. PMID: 18812438
    15. Translational control by PNPase does not play a role in autoregulation of PNP RNA. PMID: 19136586
    16. Data show that introducing the pnp gene encoding PNPase rescues defects in growth and RNA quality of the pnp mutant cells. PMID: 19219992

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  • 亚细胞定位：
    Cytoplasm. Note=Has also been isolated in association with the inner membrane.
  • 蛋白家族：
    Polyribonucleotide nucleotidyltransferase family
  • 数据库链接：

    KEGG: ecj:JW5851

    STRING: 316385.ECDH10B_3337