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  4. Recombinant Crotalus durissus terrificus Crotoxin acid chain

Recombinant Crotalus durissus terrificus Crotoxin acid chain

  • 货号：
    CSB-YP357508DYD
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP357508DYD
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP357508DYD-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP357508DYD
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP357508DYD
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Phospholipase A2 homolog crotoxin acid subunit CA; CTX subunit CA; Crotapotin) [Cleaved into: Crotoxin chain alpha CA1; CA2 and CA4; Crotoxin chain alpha CA3; Crotoxin chain beta CA2; CA3 and CA4; Crotoxin chain beta CA1; Crotoxin chain gamma; Analgesic peptide; Crotalphine)]
  • 种属：
    Crotalus durissus terrificus (South American rattlesnake)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    38-78
  • 氨基酸序列
    SSY GCYCGAGGQG WPQDASDRCC FEHDCCYAKL TGCDPTTD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    CAalpha-CAbeta-CAgamma: The acidic subunit of crotoxin (CA) is a heterotrimer of three disulfide-linked chains generated by post-translational maturation of a PLA2-like precursor. CA has no PLA2 activity and is not neurotoxic by itself, but plays several important functions in the crotoxin complex by increasing the lethal potency of the uncomplexed CB subunit. It acts by physically occluding the hydrophobic interfacial binding surface (IBS) of CB. This effect decreases the adsorption of CB to phospholipid membranes, targeting the crotoxin complex to reach the specific presynaptic receptor (R48) at the neuromuscular junction. It also prevents the formation of the reactive CB dimer. Moreover, the CA subunit inhibits the catalytic activity by partially masking the catalytic site of CB and inhibits its anticoagulant activity.; Heterodimer CA-CB: Crotoxin is a potent presynaptic neurotoxin that possesses phospholipase A2 (PLA2) activity and exerts a lethal action by blocking neuromuscular transmission. It consists of a non-covalent association of a basic and weakly toxic PLA2 subunit (CBa2, CBb, CBc, or CBd), with a small acidic, non-enzymatic and non-toxic subunit (CA1, CA2, CA3 or CA4). The complex acts by binding to a specific 48-kDa protein (R48/CAPT) receptor located on presynaptic membranes, forming a transient ternary complex CA-CB-R48, followed by dissociation of the CA-CB complex and release of the CA subunit. At equilibrium, only the CB subunits remain associated with the specific crotoxin receptor. In addition to neurotoxicity, crotoxin has been found to exert myotoxicity, nephrotoxicity, and cardiovascular toxicity. Moreover, anti-inflammatory, immunomodulatory, anti-tumor and analgesic effects of crotoxin have also been reported.; found in the venom as a monomer and stabilized by one disulfide bond (Cys-131 and Cys-138). This peptide induces potent antinociceptive effects in acute and chronic pain models. This effect is mediated by the release of peripheral dynorphin A, an endogenous agonist of kappa-opioid receptors, and this release is dependent on cannabinoid receptor CB2 activation.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Phospholipase A2 family, Group II subfamily, D49 sub-subfamily
  • 组织特异性：
    Expressed by the venom gland.