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  4. Recombinant Bacillus subtilis Serine hydroxymethyltransferase (glyA)

Recombinant Bacillus subtilis Serine hydroxymethyltransferase (glyA)

  • 货号：
    CSB-YP021274BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021274BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021274BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021274BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021274BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    glyA
  • Uniprot No.：
  • 别名：
    glyA; glyC; BSU36900; ipc-34dSerine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-415
  • 氨基酸序列
    MKHLPAQDEQ VFNAIKNERE RQQTKIELIA SENFVSEAVM EAQGSVLTNK YAEGYPGKRY YGGCEHVDVV EDIARDRAKE IFGAEHVNVQ PHSGAQANMA VYFTILEQGD TVLGMNLSHG GHLTHGSPVN FSGVQYNFVE YGVDKETQYI DYDDVREKAL AHKPKLIVAG ASAYPRTIDF KKFREIADEV GAYFMVDMAH IAGLVAAGLH PNPVPYADFV TTTTHKTLRG PRGGMILCRE EFGKKIDKSI FPGIQGGPLM HVIAAKAVSF GEVLQDDFKT YAQNVISNAK RLAEALTKEG IQLVSGGTDN HLILVDLRSL GLTGKVAEHV LDEIGITSNK NAIPYDPEKP FVTSGIRLGT AAVTSRGFDG DALEEVGAII ALALKNHEDE GKLEEARQRV AALTDKFPLY KELDY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.
  • 基因功能参考文献：
    1. low temperatures, using experimental and comparative in silico approaches. These structural and functional studies of SHMTs purified from extremophilic organisms can help to understand the peculiarities of the enzyme activity at extreme temperatures. PMID: 23841096
    2. the pyridoxal 5'- phosphate-binding domain of serine hydroxymethyltransferase is an independent domain, which behaves as a single cooperative unfolding unit under equilibrium conditions PMID: 18483062
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    SHMT family
  • 数据库链接：

    KEGG: bsu:BSU36900

    STRING: 224308.Bsubs1_010100019951